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PMID: 1898351 Published · ppublish English Comparative Study Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S.

Differential rates of conversion of rat proinsulins I and II. Evidence for slow cleavage at the B-chain/C-peptide junction of proinsulin II.

The Biochemical journal ·Vol. 278 ( Pt 3) ·1991-09-15 ·Pages 621-5

Sizonenko SV, Halban PA

Abstract

Rat proinsulin I is converted into insulin more rapidly than is proinsulin II. To study this further, rat islets were labelled (10 min) and conversion kinetics of the labelled proinsulins were monitored during a 120 min chase. Proinsulins, conversion intermediates and both insulins were separated by h.p.l.c. The accumulation of des-64,65-(split proinsulin II) during the chase suggests that the B-chain/C-peptide junction of proinsulin II is cleaved more slowly than the equivalent site on proinsulin I. This accounts for the differential kinetics of conversion of proinsulins I and II, and is presumed to be caused by one (or more) of the amino acid replacements which distinguish the two proinsulins.

MeSH Terms
Animals C-Peptide/metabolism Carboxypeptidase B Carboxypeptidases/metabolism Chromatography, High Pressure Liquid Insulin/metabolism Islets of Langerhans/metabolism Kinetics Male Proinsulin/metabolism Rats Rats, Inbred Strains Trypsin/metabolism
Chemicals
C-Peptide Insulin Proinsulin Carboxypeptidases Carboxypeptidase B Trypsin
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Sizonenko S V
Laboratories de Recherche Louis Jeantet, Centre Médical Universitaire, Geneva, Switzerland.
Halban P A
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36 references, click to expand
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Article Info
Journal
The Biochemical journal
Abbr.
Biochem J
ISSN
0264-6021
DOI
10.1042/bj2780621
Published
1991-09-15
Pages
621-5
Language
English
Region
England
NLM ID
2984726R
PMCID
PMC1151392
Subset
IM
Grants
NIDDK NIH HHS · DK 35292 · United States
External Links
PubMed source DOI: 10.1042/bj2780621 PMC Full Text View journal details
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