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PMID: 1898929 Published · ppublish English Comparative Study Journal Article Research Support, Non-U.S. Gov't

Protein secretion in Pseudomonas aeruginosa: the xcpA gene encodes an integral inner membrane protein homologous to Klebsiella pneumoniae secretion function protein PulO.

Journal of bacteriology ·Vol. 173 ·No. 2 ·1991-01-00 ·Pages 479-86

Bally M, Ball G, Badere A, Lazdunski A

Abstract

xcp mutations have pleiotropic effects on the secretion of proteins in Pseudomonas aeruginosa PAO. The nucleotide sequence of a 1.2-kb DNA fragment that complements the xcp-1 mutation has been determined. Sequence analysis shows the xcpA gene product to be a 31.8-kDa polypeptide, with a highly hydrophobic character. This is consistent with a localization in the cytoplasmic membrane in P. aeruginosa, determined after specific expression of the xcpA gene under control of the T7 phi 10 promoter. A very strong homology was found between XcpA and PulO, a membrane protein required for pullulanase secretion in Klebsiella pneumoniae. This suggests the existence of a signal sequence-dependent secretion process common to these two unrelated gram-negative bacteria.

Related Genes
PulO xcpA
MeSH Terms
Amino Acid Sequence Base Sequence Cloning, Molecular Escherichia coli/genetics Genes, Bacterial Klebsiella pneumoniae/genetics,metabolism Membrane Proteins/genetics Molecular Sequence Data Plasmids Pseudomonas aeruginosa/genetics,metabolism Restriction Mapping Sequence Homology, Nucleic Acid
Chemicals
Membrane Proteins
Authors & Affiliations
4 authors, click to expand affiliations / ORCID
Bally M
Laboratoire de Chimie Bactérienne, Centre National de la Recherche Scientifique, Marseille, France.
Ball G
Badere A
Lazdunski A
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Article Info
Journal
Journal of bacteriology
Abbr.
J Bacteriol
ISSN
0021-9193
DOI
10.1128/jb.173.2.479-486.1991
Published
1991-01-00
Pages
479-86
Language
English
Region
United States
NLM ID
2985120R
PMCID
PMC207036
Subset
IM
Databases
GENBANK
M38720, M61096, M64671, M74478, M74479, M74480, M74481, M74482, M74483, M74484, S45931
External Links
PubMed source DOI: 10.1128/jb.173.2.479-486.1991 PMC Full Text View journal details
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