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PMID: 1899668 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S.

Spatial and temporal control of nonmuscle myosin localization: identification of a domain that is necessary for myosin filament disassembly in vivo.

The Journal of cell biology ·Vol. 112 ·No. 4 ·1991-02-00 ·Pages 677-88

Egelhoff TT, Brown SS, Spudich JA

Abstract

Myosin null mutants of Dictyostelium are defective for cytokinesis, multicellular development, and capping of surface proteins. We have used these cells as transformation recipients for an altered myosin heavy chain gene that encodes a protein bearing a carboxy-terminal 34-kD truncation. This truncation eliminates threonine phosphorylation sites previously shown to control filament assembly in vitro. Despite restoration of growth in suspension, development, and ability to cap cell surface proteins, these delta C34-truncated myosin transformants display severe cytoskeletal abnormalities, including excessive localization of the truncated myosin to the cortical cytoskeleton, impaired cell shaped dynamics, and a temporal defect in myosin dissociation from beneath capped surface proteins. These data demonstrate that the carboxy-terminal domain of myosin plays a critical role in regulating the disassembly of the protein from contractile structures in vivo.

MeSH Terms
Cell Compartmentation Dictyostelium/genetics,metabolism Fungal Proteins/chemistry,genetics,metabolism Mutagenesis, Site-Directed Myosins/chemistry,genetics,metabolism Phosphorylation Structure-Activity Relationship Threonine/metabolism Transfection
Chemicals
Fungal Proteins Threonine Myosins
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Egelhoff T T
Department of Cell Biology, Stanford University School of Medicine, California.
Brown S S
Spudich J A
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36 references, click to expand
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Article Info
Journal
The Journal of cell biology
Abbr.
J Cell Biol
ISSN
0021-9525
DOI
10.1083/jcb.112.4.677
Published
1991-02-00
Pages
677-88
Language
English
Region
United States
NLM ID
0375356
PMCID
PMC2288861
Subset
IM
Grants
PHS HHS · G.M. 30387 · United States
External Links
PubMed source DOI: 10.1083/jcb.112.4.677 PMC Full Text View journal details
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