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PMID: 18996898 Published · ppublish English Journal Article Research Support, N.I.H., Extramural

Efficient processing of TFO-directed psoralen DNA interstrand crosslinks by the UvrABC nuclease.

Nucleic acids research ·Vol. 36 ·No. 22 ·2008-12-00 ·Pages 7136-45

Christensen LA, Wang H, Van Houten B, Vasquez KM

Abstract

Photoreactive psoralens can form interstrand crosslinks (ICLs) in double-stranded DNA. In eubacteria, the endonuclease UvrABC plays a key role in processing psoralen ICLs. Psoralen-modified triplex-forming oligonucleotides (TFOs) can be used to direct ICLs to specific genomic sites. Previous studies of pyrimidine-rich methoxypsoralen-modified TFOs indicated that the TFO inhibits cleavage by UvrABC. Because different chemistries may alter the processing of TFO-directed ICLs, we investigated the effect of another type of triplex formed by purine-rich TFOs on the processing of 4'-(hydroxymethyl)-4,5',8-trimethylpsoralen (HMT) ICLs by the UvrABC nuclease. Using an HMT-modified TFO to direct ICLs to a specific site, we found that UvrABC made incisions on the purine-rich strand of the duplex approximately 3 bases from the 3'-side and approximately 9 bases from the 5'-side of the ICL, within the TFO-binding region. In contrast to previous reports, the UvrABC nuclease cleaved the TFO-directed psoralen ICL with a greater efficiency than that of the psoralen ICL alone. Furthermore, the TFO was dissociated from its duplex binding site by UvrA and UvrB. As mutagenesis by TFO-directed ICLs requires nucleotide excision repair, the efficient processing of these lesions supports the use of triplex technology to direct DNA damage for genome modification.

MeSH Terms
Cross-Linking Reagents/chemistry DNA/chemistry,metabolism Endodeoxyribonucleases/metabolism Escherichia coli Proteins/metabolism Oligonucleotides/chemistry,metabolism Trioxsalen/analogs & derivatives,chemistry
Chemicals
Cross-Linking Reagents Escherichia coli Proteins Oligonucleotides triplex DNA hydroxymethyltrioxsalen DNA Endodeoxyribonucleases endodeoxyribonuclease uvrABC Trioxsalen
Authors & Affiliations
4 authors, click to expand affiliations / ORCID
Christensen Laura A
Department of Carcinogenesis, Science Park-Research Division, University of Texas MD Anderson Cancer Center, Smithville, TX, USA.
Wang Hong
Van Houten Bennett
Vasquez Karen M
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Article Info
Journal
Nucleic acids research
Abbr.
Nucleic Acids Res
ISSN
1362-4962
DOI
10.1093/nar/gkn880
Published
2008-12-00
Epub
2008-00-07
Pages
7136-45
Language
English
Region
England
NLM ID
0411011
PMCID
PMC2602775
Subset
IM
Grants
NCI NIH HHS · CA093729 · United States
NCI NIH HHS · CA097175 · United States
NIEHS NIH HHS · ES007784 · United States
NCI NIH HHS · P01 CA097175 · United States
External Links
PubMed source DOI: 10.1093/nar/gkn880 PMC Full Text View journal details
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