Limiting lumens: a new role for Cdc42.
The formation of a single lumen is a necessary step in the formation of biological tubes. Different tissues have developed diverse ways to form their lumens. In this issue, Jaffe et al. (Jaffe, A.B., N. Kaji, J. Durgan, and A. Hall. 2008. J. Cell Biol. 183:625-633) report the development of an in vitro system for studying lumen formation that is driven by fluid transport, recapitulating intestinal lumen formation. Effective ion and fluid transport requires both cell polarity and proper tissue organization. Surprisingly, polarization of cells in this three-dimensional system does not require Cdc42. Instead, Cdc42 prevents formation of multiple lumens by orienting cell divisions and directing apical membrane biogenesis.
19 references, click to expand
-
Endothelial tubes assemble from intracellular vacuoles in vivo.Nature. 2006 Jul 27;442(7101):453-6 PMID: 16799567
-
Cdc42--the centre of polarity.J Cell Sci. 2004 Mar 15;117(Pt 8):1291-300 PMID: 15020669
-
Cdc42 controls spindle orientation to position the apical surface during epithelial morphogenesis.J Cell Biol. 2008 Nov 17;183(4):625-33 PMID: 19001128
-
Sequential pulses of apical epithelial secretion and endocytosis drive airway maturation in Drosophila.Dev Cell. 2007 Aug;13(2):214-25 PMID: 17681133
-
Orientation of spindle axis and distribution of plasma membrane proteins during cell division in polarized MDCKII cells.J Cell Biol. 1994 Sep;126(6):1509-26 PMID: 8089182
-
Tumor necrosis factor-related apoptosis-inducing ligand (TRAIL) is required for induction of autophagy during lumen formation in vitro.Proc Natl Acad Sci U S A. 2004 Mar 9;101(10):3438-43 PMID: 14993595
-
Cdc42 is not essential for filopodium formation, directed migration, cell polarization, and mitosis in fibroblastoid cells.Mol Biol Cell. 2005 Oct;16(10):4473-84 PMID: 16014609
-
Lumen formation during mammary epithelial morphogenesis: insights from in vitro and in vivo models.Cell Cycle. 2008 Jan 1;7(1):57-62 PMID: 18196964
-
Electron microscopic observations on the maintenance of the tight junction during cell division in the epithelium of the mouse small intestine.Cell Struct Funct. 1992 Feb;17(1):27-37 PMID: 1586965
-
Polarization of the C. elegans embryo by RhoGAP-mediated exclusion of PAR-6 from cell contacts.Science. 2008 Jun 27;320(5884):1771-4 PMID: 18583611
-
CDC-42 controls early cell polarity and spindle orientation in C. elegans.Curr Biol. 2001 Apr 3;11(7):482-8 PMID: 11412997
-
PAR-6 is a conserved PDZ domain-containing protein that colocalizes with PAR-3 in Caenorhabditis elegans embryos.Development. 1999 Jan;126(1):127-35 PMID: 9834192
-
Planar spindle orientation and asymmetric cytokinesis in the mouse small intestine.J Histochem Cytochem. 2007 Nov;55(11):1173-80 PMID: 17712178
-
The role of apoptosis in creating and maintaining luminal space within normal and oncogene-expressing mammary acini.Cell. 2002 Oct 4;111(1):29-40 PMID: 12372298
-
PTEN-mediated apical segregation of phosphoinositides controls epithelial morphogenesis through Cdc42.Cell. 2007 Jan 26;128(2):383-97 PMID: 17254974
-
Cdc42 is crucial for the establishment of epithelial polarity during early mammalian development.Dev Dyn. 2007 Oct;236(10):2767-78 PMID: 17849438
-
The agrin/perlecan-related protein eyes shut is essential for epithelial lumen formation in the Drosophila retina.Dev Cell. 2006 Oct;11(4):483-93 PMID: 17011488
-
Genetic control of single lumen formation in the zebrafish gut.Nat Cell Biol. 2007 Aug;9(8):954-60 PMID: 17632505
-
Tight junctions in early amphibian development: detection of junctional cingulin from the 2-cell stage and its localization at the boundary of distinct membrane domains in dividing blastomeres in low calcium.Dev Dyn. 1996 Sep;207(1):104-13 PMID: 8875080
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