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PMID: 1901232 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S.

Two-dimensional crystals of streptavidin on biotinylated lipid layers and their interactions with biotinylated macromolecules.

Biophysical journal ·Vol. 59 ·No. 2 ·1991-02-00 ·Pages 387-96

Darst SA, Ahlers M, Meller PH, Kubalek EW, Blankenburg R, Ribi HO, Ringsdorf H, Kornberg RD

Abstract

Streptavidin forms two-dimensional crystals when specifically bound to layers of biotinylated lipids at the air/water interface. The three-dimensional structure of streptavidin determined from the crystals by electron crystallography corresponds well with the structure determined by x-ray crystallography. Comparison of the electron and x-ray crystallographic structures reveals the occurrence of free biotin-binding sites on the surface of the two-dimensional crystals facing the aqueous solution. The free biotin-binding sites could be specifically labeled with biotinylated ferritin. The streptavidin/biotinylated lipid system may provide a general approach for the formation of two-dimensional crystals of biotinylated macromolecules.

MeSH Terms
Bacterial Proteins/chemistry,metabolism,ultrastructure Binding Sites Biotin/metabolism Fluorescein-5-isothiocyanate Fluoresceins Fluorescent Dyes Liposomes Microscopy, Electron Models, Structural Molecular Conformation Protein Binding Protein Conformation Streptavidin Thiocyanates
Chemicals
Bacterial Proteins Fluoresceins Fluorescent Dyes Liposomes Thiocyanates Biotin Streptavidin Fluorescein-5-isothiocyanate
Authors & Affiliations
8 authors, click to expand affiliations / ORCID
Darst S A
Department of Cell Biology, Stanford University School of Medicine, California 94305.
Ahlers M
Meller P H
Kubalek E W
Blankenburg R
Ribi H O
Ringsdorf H
Kornberg R D
References (11)
11 references, click to expand
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Article Info
Journal
Biophysical journal
Abbr.
Biophys J
ISSN
0006-3495
DOI
10.1016/S0006-3495(91)82232-9
Published
1991-02-00
Pages
387-96
Language
English
Region
United States
NLM ID
0370626
PMCID
PMC1281155
Subset
IM
Grants
NIAID NIH HHS · AI21144 · United States
NIGMS NIH HHS · GM30387 · United States
External Links
PubMed source DOI: 10.1016/S0006-3495(91)82232-9 PMC Full Text View journal details
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