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PMID: 1901659 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Generation of sensory neurons is stimulated by leukemia inhibitory factor.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 88 ·No. 8 ·1991-04-15 ·Pages 3498-501

Murphy M, Reid K, Hilton DJ, Bartlett PF

Abstract

The processes that regulate the development of peripheral neurons from their precursors in the embryonic neural crest are essentially unknown. In this report, we show that leukemia inhibitory factor stimulates the generation of neurons in cultures of mouse neural crest. These neurons have the morphology of sensory neurons and contain neuropeptides found in mammalian sensory neurons. Consistent with these neurons being of the sensory lineage is the finding that they arise from nondividing precursors within the neural crest. In addition, we show that leukemia inhibitory factor supports the generation and/or maturation of sensory neurons in cultures of cells obtained from embryonic dorsal root ganglia. In cultures of postnatal dorsal root ganglia, which contain mature sensory neurons, leukemia inhibitory factor acts directly as a survival molecule on the majority of neurons.

MeSH Terms
Animals Cell Differentiation/drug effects Cell Division/drug effects Cells, Cultured Ganglia, Spinal/cytology Growth Inhibitors In Vitro Techniques Interleukin-6 Leukemia Inhibitory Factor Lymphokines/pharmacology Mice Neural Crest/cytology Neurons, Afferent/cytology
Chemicals
Growth Inhibitors Interleukin-6 Leukemia Inhibitory Factor Lif protein, mouse Lymphokines
Authors & Affiliations
4 authors, click to expand affiliations / ORCID
Murphy M
Walter and Eliza Hall Institute of Medical Research, Royal Melbourne Hospital, Victoria, Australia.
Reid K
Hilton D J
Bartlett P F
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Article Info
Journal
Proceedings of the National Academy of Sciences of the United States of America
Abbr.
Proc Natl Acad Sci U S A
ISSN
0027-8424
DOI
10.1073/pnas.88.8.3498
Published
1991-04-15
Pages
3498-501
Language
English
Region
United States
NLM ID
7505876
PMCID
PMC51475
Subset
IM
External Links
PubMed source DOI: 10.1073/pnas.88.8.3498 PMC Full Text View journal details
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