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PMID: 1903538 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S.

Human X-box-binding protein 1 is required for the transcription of a subset of human class II major histocompatibility genes and forms a heterodimer with c-fos.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 88 ·No. 10 ·1991-05-15 ·Pages 4309-12

Ono SJ, Liou HC, Davidon R, Strominger JL, Glimcher LH

Abstract

A complementary DNA encoding a member of the leucine-zipper class of proteins (human X-box-binding protein, hXBP-1) that binds to the 3' end of the conserved X box (X2) of the HLA-DRA major histocompatibility complex gene was recently described. Further gel-retardation analysis has demonstrated that hXBP-1 also binds to HLA-DPB X2 but not to other X2 sequences. Transient transfection of a mammalian expression vector with the hXBP-1 cDNA inserted in the antisense orientation represses the surface expression of HLA-DR and HLA-DP in Raji cells. Cotransfection of the antisense hXBP-1 vector with a HLA-DRA/chloramphenicol acetyltransferase (but not a HLA-DQB/chloramphenicol acetyltransferase) reporter plasmid decreases chloramphenicol acetyltransferase activity in Raji cells and in gamma-interferon-treated HeLa cells relative to cells cotransfected with a control antisense vector. Moreover, hXBP-1 is shown to form a stable heterodimer with the product of the c-fos protooncogene. These data suggest that the hXBP-1 c-fos heterodimer is critical for the transcription of a subset of the human class II major histocompatibility complex genes and that the regulatory mechanisms for the different class II genes are distinct.

MeSH Terms
Antisense Elements (Genetics) Base Sequence DNA/genetics DNA-Binding Proteins/genetics,physiology Gene Expression Regulation Genes, MHC Class II HLA-DP Antigens/genetics HLA-DR Antigens/genetics Humans Immunosorbent Techniques Macromolecular Substances Molecular Sequence Data Proto-Oncogene Proteins/metabolism Proto-Oncogene Proteins c-fos Recombinant Fusion Proteins/metabolism Regulatory Factor X Transcription Factors Transcription Factors/genetics,physiology Transcription, Genetic Transfection
Chemicals
Antisense Elements (Genetics) DNA-Binding Proteins HLA-DP Antigens HLA-DR Antigens Macromolecular Substances Proto-Oncogene Proteins Proto-Oncogene Proteins c-fos Recombinant Fusion Proteins Regulatory Factor X Transcription Factors Transcription Factors DNA
Authors & Affiliations
5 authors, click to expand affiliations / ORCID
Ono S J
Department of Biochemistry and Molecular Biology, Harvard University, Cambridge, MA 02138.
Liou H C
Davidon R
Strominger J L
Glimcher L H
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Article Info
Journal
Proceedings of the National Academy of Sciences of the United States of America
Abbr.
Proc Natl Acad Sci U S A
ISSN
0027-8424
DOI
10.1073/pnas.88.10.4309
Published
1991-05-15
Pages
4309-12
Language
English
Region
United States
NLM ID
7505876
PMCID
PMC51648
Subset
IM
Grants
NCI NIH HHS · CA-47554 · United States
NIDDK NIH HHS · DK-30241 · United States
NIGMS NIH HHS · GM36864 · United States
External Links
PubMed source DOI: 10.1073/pnas.88.10.4309 PMC Full Text View journal details
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