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PMID: 1903914 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S.

Hydrolysis of carbaryl by a Pseudomonas sp. and construction of a microbial consortium that completely metabolizes carbaryl.

Applied and environmental microbiology ·Vol. 57 ·No. 3 ·1991-03-00 ·Pages 744-50

Chapalamadugu S, Chaudhry GR

Abstract

Two Pseudomonas spp. (isolates 50552 and 50581) isolated from soil degraded 1-naphthol and carbaryl, an N-methylcarbamate pesticide, respectively. They utilized these compounds as a sole source of carbon. 1-Naphthol was completely metabolized to CO2 by the isolate 50552, while the carbaryl was first hydrolyzed to 1-naphthol and then converted into a brown-colored compound by the isolate 50581. The colored metabolite was not degraded, but 1-naphthol produced by the isolate 50581 during the exponential phase of growth was metabolized by the isolate 50552. The two isolates were used to construct a bacterial consortium which completely catabolized carbaryl to CO2. No metabolite was detected in the cell cultures of the consortium. The isolate 50581 harbored a 50-kb plasmid pCD1, while no plasmid was detected in the isolate 50552. The isolated bacteria individually or as a consortium may be used for detoxification of certain industrial and agricultural wastes.

MeSH Terms
Biodegradation, Environmental Carbaryl/metabolism Hydrolysis Naphthols/metabolism Plasmids Pseudomonas/genetics,metabolism Soil Microbiology
Chemicals
Naphthols 1-naphthol Carbaryl
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Chapalamadugu S
Department of Biological Sciences, Oakland University, Rochester, Michigan 48309-4401.
Chaudhry G R
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Article Info
Journal
Applied and environmental microbiology
Abbr.
Appl Environ Microbiol
ISSN
0099-2240
DOI
10.1128/aem.57.3.744-750.1991
Published
1991-03-00
Pages
744-50
Language
English
Region
United States
NLM ID
7605801
PMCID
PMC182789
Subset
IM
Grants
NCRR NIH HHS · BRSG 50RR713 · United States
External Links
PubMed source DOI: 10.1128/aem.57.3.744-750.1991 PMC Full Text View journal details
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