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PMID: 19041756 Published · ppublish English Journal Article Research Support, N.I.H., Extramural Research Support, Non-U.S. Gov't

A new component in synaptic plasticity: upregulation of kinesin in the neurons of the gill-withdrawal reflex.

Cell ·Vol. 135 ·No. 5 ·2008-11-28 ·Pages 960-73

Puthanveettil SV, Monje FJ, Miniaci MC, Choi YB, Karl KA, Khandros E, Gawinowicz MA, Sheetz MP, Kandel ER

Abstract

To explore how gene products, required for the initiation of synaptic growth, move from the cell body of the sensory neuron to its presynaptic terminals, and from the cell body of the motor neuron to its postsynaptic dendritic spines, we have investigated the anterograde transport machinery in both the sensory and motor neurons of the gill-withdrawal reflex of Aplysia. We found that the induction of long-term facilitation (LTF) by repeated applications of serotonin, a modulatory transmitter released during learning in Aplysia, requires upregulation of kinesin heavy chain (KHC) in both pre- and postsynaptic neurons. Indeed, upregulation of KHC in the presynaptic neurons alone is sufficient for the induction of LTF. However, KHC is not required for the persistence of LTF. Thus, in addition to transcriptional activation in the nucleus and local protein synthesis at the synapse, our studies have identified a third component critical for long-term learning-related plasticity: the coordinated upregulation of kinesin-mediated transport.

MeSH Terms
Animals Aplysia/physiology Gills/physiology Kinesins/physiology Neuronal Plasticity Neurons/physiology Synapses/physiology Up-Regulation
Chemicals
Kinesins
Authors & Affiliations
9 authors, click to expand affiliations / ORCID
Puthanveettil Sathyanarayanan V
Department of Neuroscience, College of Physicians and Surgeons, Columbia University, 1051 Riverside Drive, New York, NY 10032, USA.
Monje Francisco J
Miniaci Maria Concetta
Choi Yun-Beom
Karl Kevin A
Khandros Eugene
Gawinowicz Mary Ann
Sheetz Michael P
Kandel Eric R
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Article Info
Journal
Cell
Abbr.
Cell
ISSN
1097-4172
DOI
10.1016/j.cell.2008.11.003
Published
2008-11-28
Pages
960-73
Language
English
Region
United States
NLM ID
0413066
PMCID
PMC2635114
Subset
IM
Grants
NINDS NIH HHS · R01 NS060762 · United States
NHGRI NIH HHS · P50 HG002806 · United States
NHGRI NIH HHS · P50 HG002806-03 · United States
NIMH NIH HHS · R01 MH075026-04 · United States
NIMH NIH HHS · R01 MH075026 · United States
Howard Hughes Medical Institute · United States
External Links
PubMed source DOI: 10.1016/j.cell.2008.11.003 PMC Full Text View journal details
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