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PMID: 1904861 Published · ppublish English Journal Article Research Support, U.S. Gov't, P.H.S.

Species-specific uptake of DNA by gonococci is mediated by a 10-base-pair sequence.

Journal of bacteriology ·Vol. 173 ·No. 12 ·1991-06-00 ·Pages 3911-3

Elkins C, Thomas CE, Seifert HS, Sparling PF

Abstract

Piliated Neisseria gonorrhoeae are known to be transformed less readily if transforming DNA competes with DNA containing the 10-bp sequence GCCGTCTGAA. It has been postulated that the 10-bp sequence is a recognition sequence which is required for efficient DNA uptake. We show that the presence of various forms of this 10-bp sequence results in increased uptake of double-stranded DNA into a DNase-resistant state and allows genetic transformation by an otherwise nontransformable plasmid.

MeSH Terms
Base Sequence Binding, Competitive DNA, Bacterial/genetics,metabolism Escherichia coli/genetics Genes, Bacterial Molecular Sequence Data Neisseria gonorrhoeae/metabolism Plasmids Species Specificity Transformation, Genetic
Chemicals
DNA, Bacterial
Authors & Affiliations
4 authors, click to expand affiliations / ORCID
Elkins C
Department of Microbiology and Immunology, University of North Carolina, Chapel Hill 27599.
Thomas C E
Seifert H S
Sparling P F
References (17)
17 references, click to expand
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Article Info
Journal
Journal of bacteriology
Abbr.
J Bacteriol
ISSN
0021-9193
DOI
10.1128/jb.173.12.3911-3913.1991
Published
1991-06-00
Pages
3911-3
Language
English
Region
United States
NLM ID
2985120R
PMCID
PMC208026
Subset
IM
Grants
NIAID NIH HHS · AI15036 · United States
NIAID NIH HHS · AI26837 · United States
NIAID NIH HHS · AI27195 · United States
External Links
PubMed source DOI: 10.1128/jb.173.12.3911-3913.1991 PMC Full Text View journal details
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