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PMID: 19066226 Published · ppublish English Journal Article Research Support, N.I.H., Extramural Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, Non-P.H.S.

AGO1-miR173 complex initiates phased siRNA formation in plants.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 105 ·No. 51 ·2008-12-23 ·Pages 20055-62

Montgomery TA, Yoo SJ, Fahlgren N, Gilbert SD, Howell MD, Sullivan CM, Alexander A, Nguyen G, Allen E, Ahn JH, Carrington JC

Abstract

MicroRNA (miRNA)-guided cleavage initiates entry of primary transcripts into the transacting siRNA (tasiRNA) biogenesis pathway involving RNA-DEPENDENT RNA POLYMERASE6, DICER-LIKE4, and SUPPRESSOR OF GENE SILENCING3. Arabidopsis thaliana TAS1 and TAS2 families yield tasiRNA that form through miR173-guided initiation-cleavage of primary transcripts and target several transcripts encoding pentatricopeptide repeat proteins and proteins of unknown function. Here, the TAS1c locus was modified to produce synthetic (syn) tasiRNA to target an endogenous transcript encoding PHYTOENE DESATURASE and used to analyze the role of miR173 in routing of transcripts through the tasiRNA pathway. miR173 was unique from other miRNAs in its ability to initiate TAS1c-based syn-tasiRNA formation. A single miR173 target site was sufficient to route non-TAS transcripts into the pathway to yield phased siRNA. We also show that miR173 functions in association with ARGONAUTE 1 (AGO1) during TAS1 and TAS2 tasiRNA formation, and we provide data indicating that the miR173-AGO1 complex possesses unique functionality that many other miRNA-AGO1 complexes lack.

MeSH Terms
Arabidopsis Proteins/genetics,metabolism,physiology Argonaute Proteins MicroRNAs/metabolism,physiology RNA, Messenger RNA, Plant/biosynthesis RNA, Small Interfering/biosynthesis,genetics
Chemicals
AGO1 protein, Arabidopsis Arabidopsis Proteins Argonaute Proteins MIRN173 microRNA, Arabidopsis MicroRNAs RNA, Messenger RNA, Plant RNA, Small Interfering
Authors & Affiliations
11 authors, click to expand affiliations / ORCID
Montgomery Taiowa A
Molecular and Cellular Biology Program, Department of Botany and Plant Pathology, Oregon State University, Corvallis, OR 97331, USA.
Yoo Seong Jeon
Fahlgren Noah
Gilbert Sunny D
Howell Miya D
Sullivan Christopher M
Alexander Amanda
Nguyen Goretti
Allen Edwards
Ahn Ji Hoon
Carrington James C
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Article Info
Journal
Proceedings of the National Academy of Sciences of the United States of America
Abbr.
Proc Natl Acad Sci U S A
ISSN
1091-6490
DOI
10.1073/pnas.0810241105
Published
2008-12-23
Epub
2008-00-09
Pages
20055-62
Language
English
Region
United States
NLM ID
7505876
PMCID
PMC2598728
Subset
IM
Grants
NIAID NIH HHS · R01 AI043288 · United States
NIAID NIH HHS · R21 AI043288 · United States
NIAID NIH HHS · R37 AI043288 · United States
NIAID NIH HHS · AI43288 · United States
Databases
GEO
GSE13605
External Links
PubMed source DOI: 10.1073/pnas.0810241105 PMC Full Text View journal details
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