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PMID: 19079251 Published · ppublish English Journal Article Research Support, N.I.H., Extramural Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, Non-P.H.S. Technical Report

Bi-stable neural state switches.

Nature neuroscience ·Vol. 12 ·No. 2 ·2009-02-00 ·Pages 229-34

Berndt A, Yizhar O, Gunaydin LA, Hegemann P, Deisseroth K

Abstract

Here we describe bi-stable channelrhodopsins that convert a brief pulse of light into a stable step in membrane potential. These molecularly engineered probes nevertheless retain millisecond-scale temporal precision. Photocurrents can be precisely initiated and terminated with different colors of light, but operate at vastly longer time scales than conventional channelrhodopsins as a result of modification at the C128 position that extends the lifetime of the open state. Because of their enhanced kinetic stability, these step-function tools are also effectively responsive to light at orders of magnitude lower intensity than wild-type channelrhodopsins. These molecules therefore offer important new capabilities for a broad range of in vivo applications.

MeSH Terms
Animals Cell Line Cells, Cultured Genetic Engineering/methods Hippocampus/cytology Humans Kidney/cytology Light Signal Transduction/physiology Mutagenesis, Site-Directed Neurons/cytology,physiology Oocytes/physiology Rats Rats, Sprague-Dawley Rhodopsin/genetics,physiology Transfection Vision, Ocular/physiology Xenopus laevis
Chemicals
Rhodopsin
Authors & Affiliations
5 authors, click to expand affiliations / ORCID
Berndt André
Institute of Biology, Experimental Biophysics, Humboldt-University, Invalidenstrasse 42, D-10115 Berlin, Germany.
Yizhar Ofer
Gunaydin Lisa A
Hegemann Peter
Deisseroth Karl
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Article Info
Journal
Nature neuroscience
Abbr.
Nat Neurosci
ISSN
1546-1726
DOI
10.1038/nn.2247
Published
2009-02-00
Epub
2008-00-08
Pages
229-34
Language
English
Region
United States
NLM ID
9809671
Subset
IM
External Links
PubMed source DOI: 10.1038/nn.2247 View journal details
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