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PMID: 19086176 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Overexpression of the tomato K+/H+ antiporter LeNHX2 confers salt tolerance by improving potassium compartmentalization.

The New phytologist ·Vol. 179 ·No. 2 ·2008-07-00 ·Pages 366-377

Rodríguez-Rosales MP, Jiang X, Gálvez FJ, Aranda MN, Cubero B, Venema K

Abstract

Here, the function of the tomato (Solanum lycopersicon) K+/H+ antiporter LeNHX2 was studied using 35S-driven gene overexpression of a histagged LeNHX2 protein in Arabidopsis thaliana and LeNHX2 gene silencing in tomato. Transgenic A. thaliana plants expressed the histagged LeNHX2 both in shoots and in roots, as assayed by western blotting. Transitory expression of a green fluorescent protein (GFP) tagged protein showed that the antiporter is present in small vesicles. Internal membrane vesicles from transgenic plants displayed enhanced K+/H+ exchange activity, confirming the K+/H+ antiporter function of this enzyme. Transgenic A. thaliana plants overexpressing the histagged tomato antiporter LeNHX2 exhibited inhibited growth in the absence of K+ in the growth medium, but were more tolerant to high concentrations of Na+ than untransformed controls. When grown in the presence of NaCl, transgenic plants contained lower concentrations of intracellular Na+, but more K+, as compared with untransformed controls. Silencing of LeNHX2 in S. lycopersicon plants produced significant inhibition of plant growth and fruit and seed production as well as increased sensitivity to NaCl. The data indicate that regulation of K+ homeostasis by LeNHX2 is essential for normal plant growth and development, and plays an important role in the response to salt stress by improving K+ accumulation.

MeSH Terms
Arabidopsis/genetics,metabolism Gene Expression Regulation, Plant/genetics,physiology Gene Silencing Lycopersicon esculentum/genetics,metabolism Plant Proteins/genetics,metabolism Plants, Genetically Modified Potassium/metabolism Potassium-Hydrogen Antiporters/genetics,metabolism Sodium Chloride/pharmacology
Chemicals
Plant Proteins Potassium-Hydrogen Antiporters Sodium Chloride Potassium
Authors & Affiliations
6 authors, click to expand affiliations / ORCID
Rodríguez-Rosales María Pilar
Department of Biochemistry and Molecular and Cell Biology of Plants, Estación Experimental del Zaidín, CSIC, 18008 Granada, Spain.
Jiang Xingyu
Department of Biochemistry and Molecular and Cell Biology of Plants, Estación Experimental del Zaidín, CSIC, 18008 Granada, Spain. | Department of Plant Biology, Instituto de Recursos Naturales y Agrobiología, CSIC, 41012 Sevilla, Spain.
Gálvez Francisco Javier
Department of Biochemistry and Molecular and Cell Biology of Plants, Estación Experimental del Zaidín, CSIC, 18008 Granada, Spain.
Aranda María Nieves
Department of Biochemistry and Molecular and Cell Biology of Plants, Estación Experimental del Zaidín, CSIC, 18008 Granada, Spain.
Cubero Beatriz
Department of Plant Biology, Instituto de Recursos Naturales y Agrobiología, CSIC, 41012 Sevilla, Spain.
Venema Kees
Department of Biochemistry and Molecular and Cell Biology of Plants, Estación Experimental del Zaidín, CSIC, 18008 Granada, Spain.
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Article Info
Journal
The New phytologist
Abbr.
New Phytol
ISSN
0028-646X
DOI
10.1111/j.1469-8137.2008.02461.x
Published
2008-07-00
Pages
366-377
Language
English
Region
England
NLM ID
9882884
Subset
IM
External Links
PubMed source DOI: 10.1111/j.1469-8137.2008.02461.x View journal details
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