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PMID: 19106376 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

A tobacco calcium-dependent protein kinase, CDPK1, regulates the transcription factor REPRESSION OF SHOOT GROWTH in response to gibberellins.

The Plant cell ·Vol. 20 ·No. 12 ·2008-12-00 ·Pages 3273-88

Ishida S, Yuasa T, Nakata M, Takahashi Y

Abstract

The homeostasis of gibberellins (GAs) is maintained by negative feedback in plants. REPRESSION OF SHOOT GROWTH (RSG) is a tobacco (Nicotiana tabacum) transcriptional activator that has been suggested to play a role in GA feedback by the regulation of GA biosynthetic enzymes. The 14-3-3 signaling proteins negatively regulate RSG by sequestering it in the cytoplasm in response to GAs. The phosphorylation on Ser-114 of RSG is essential for 14-3-3 binding of RSG. Here, we identified tobacco Ca(2+)-dependent protein kinase (CDPK1) as an RSG kinase that promotes 14-3-3 binding to RSG by phosphorylation of Ser-114 of RSG. CDPK1 interacts with RSG in a Ca(2+)-dependent manner in vivo and in vitro and specifically phosphorylates Ser-114 of RSG. Inhibition of CDPK repressed the GA-induced phosphorylation of Ser-114 of RSG and the GA-induced nuclear export of RSG. Overexpression of CDPK1 inhibited the feedback regulation of a GA 20-oxidase gene and resulted in sensitization to the GA biosynthetic inhibitor. Our results suggest that CDPK1 decodes the Ca(2+) signal produced by GAs and regulates the intracellular localization of RSG.

MeSH Terms
14-3-3 Proteins/metabolism,physiology Calcium-Binding Proteins/genetics,metabolism,physiology Gibberellins/pharmacology Immunoblotting Immunoprecipitation Microscopy, Fluorescence Molecular Sequence Data Phosphorylation/drug effects Plant Proteins/genetics,metabolism,physiology Plants, Genetically Modified/drug effects,genetics,metabolism Protein Binding Protein Kinases/genetics,metabolism,physiology Reverse Transcriptase Polymerase Chain Reaction Signal Transduction/drug effects,genetics Tobacco/drug effects,genetics,metabolism
Chemicals
14-3-3 Proteins Calcium-Binding Proteins Gibberellins Plant Proteins Protein Kinases
Authors & Affiliations
4 authors, click to expand affiliations / ORCID
Ishida Sarahmi
Department of Biological Sciences, Graduate School of Science, University of Tokyo, Hongo, Bunkyo-ku, Tokyo 113-0033, Japan.
Yuasa Takashi
Nakata Masaru
Takahashi Yohsuke
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Article Info
Journal
The Plant cell
Abbr.
Plant Cell
ISSN
1040-4651
DOI
10.1105/tpc.107.057489
Published
2008-12-00
Epub
2008-00-23
Pages
3273-88
Language
English
Region
England
NLM ID
9208688
PMCID
PMC2630431
Subset
IM
Databases
GENBANK
AB012856, AB040471, AB071967, AF072908, AJ344154
External Links
PubMed source DOI: 10.1105/tpc.107.057489 PMC Full Text View journal details
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