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PMID: 19128792 Published · ppublish English Journal Article Research Support, N.I.H., Extramural Research Support, Non-U.S. Gov't

Tissue damage-induced intestinal stem cell division in Drosophila.

Cell stem cell ·Vol. 4 ·No. 1 ·2009-01-09 ·Pages 49-61

Amcheslavsky A, Jiang J, Ip YT

Abstract

Stem cell division is essential for tissue integrity during growth, aging, and pathogenic assaults. Adult gastrointestinal tract encounters numerous stimulations, and impaired tissue regeneration may lead to inflammatory diseases and cancer. Intestinal stem cells in adult Drosophila have recently been identified and shown to replenish the various cell types within the midgut. However, it is not known whether these intestinal stem cells can respond to environmental challenges. By feeding dextran sulfate sodium and bleomycin to flies and by expressing apoptotic proteins, we show that Drosophila intestinal stem cells can increase the rate of division in response to tissue damage. Moreover, if tissue damage results in epithelial cell loss, the newly formed enteroblasts can differentiate into mature epithelial cells. By using this newly established system of intestinal stem cell proliferation and tissue regeneration, we find that the insulin receptor signaling pathway is required for intestinal stem cell division.

MeSH Terms
Animals Apoptosis/drug effects Basement Membrane/drug effects,metabolism,pathology Bleomycin/pharmacology Cell Count Cell Differentiation/drug effects Cell Division/drug effects Cell Lineage/drug effects Cell Proliferation/drug effects Dextran Sulfate/administration & dosage,pharmacology Drosophila Proteins/metabolism Drosophila melanogaster/cytology,drug effects,metabolism Enterocytes/drug effects,metabolism,pathology Insulin/metabolism Intestinal Mucosa/metabolism Intestines/cytology,drug effects,pathology Models, Biological Receptor, Insulin/metabolism Signal Transduction/drug effects Stem Cells/cytology,drug effects,metabolism
Chemicals
Drosophila Proteins Insulin Bleomycin Dextran Sulfate Receptor, Insulin
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Amcheslavsky Alla
Program in Molecular Medicine, University of Massachusetts Medical School, Worcester, MA 01605, USA.
Jiang Jin
Ip Y Tony
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Article Info
Journal
Cell stem cell
Abbr.
Cell Stem Cell
ISSN
1875-9777
DOI
10.1016/j.stem.2008.10.016
Published
2009-01-09
Pages
49-61
Language
English
Region
United States
NLM ID
101311472
PMCID
PMC2659574
Subset
IM
Grants
NIGMS NIH HHS · R01 GM053269-13 · United States
NIGMS NIH HHS · R01 GM067045 · United States
NIDDK NIH HHS · DK32520 · United States
NIDDK NIH HHS · R21 DK075545 · United States
NIGMS NIH HHS · R01 GM061269 · United States
NIGMS NIH HHS · R01 GM053269 · United States
NIGMS NIH HHS · GM53269 · United States
NIDDK NIH HHS · DK75575 · United States
NIDDK NIH HHS · P30 DK032520 · United States
NIDDK NIH HHS · R01 DK083450 · United States
NIDDK NIH HHS · R21 DK075545-02 · United States
External Links
PubMed source DOI: 10.1016/j.stem.2008.10.016 PMC Full Text View journal details
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