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PMID: 19136372 Published · ppublish English Journal Article Research Support, N.I.H., Extramural Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, Non-P.H.S.

Rapid DNA methylation changes after exposure to traffic particles.

American journal of respiratory and critical care medicine ·Vol. 179 ·No. 7 ·2009-04-01 ·Pages 572-8

Baccarelli A, Wright RO, Bollati V, Tarantini L, Litonjua AA, Suh HH, Zanobetti A, Sparrow D, Vokonas PS, Schwartz J

Abstract

Exposure to particulate air pollution has been related to increased hospitalization and death, particularly from cardiovascular disease. Lower blood DNA methylation content is found in processes related to cardiovascular outcomes, such as oxidative stress, aging, and atherosclerosis. We evaluated whether particulate pollution modifies DNA methylation in heavily methylated sequences with high representation throughout the human genome. We measured DNA methylation of long interspersed nucleotide element (LINE)-1 and Alu repetitive elements by quantitative polymerase chain reaction-pyrosequencing of 1,097 blood samples from 718 elderly participants in the Boston area Normative Aging Study. We used covariate-adjusted mixed models to account for within-subject correlation in repeated measures. We estimated the effects on DNA methylation of ambient particulate pollutants (black carbon, particulate matter with aerodynamic diameter < or = 2.5 microm [PM2.5], or sulfate) in multiple time windows (4 h to 7 d) before the examination. We estimated standardized regression coefficients (beta) expressing the fraction of a standard deviation change in DNA methylation associated with a standard deviation increase in exposure. Repetitive element DNA methylation varied in association with time-related variables, such as day of the week and season. LINE-1 methylation decreased after recent exposure to higher black carbon (beta = -0.11; 95% confidence interval [CI], -0.18 to -0.04; P = 0.002) and PM2.5 (beta = -0.13; 95% CI, -0.19 to -0.06; P < 0.001 for the 7-d moving average). In two-pollutant models, only black carbon, a tracer of traffic particles, was significantly associated with LINE-1 methylation (beta = -0.09; 95% CI, -0.17 to -0.01; P = 0.03). No association was found with Alu methylation (P > 0.12). We found decreased repeated-element methylation after exposure to traffic particles. Whether decreased methylation mediates exposure-related health effects remains to be determined.

MeSH Terms
Aged Aged, 80 and over Alu Elements/drug effects DNA Methylation/drug effects Epigenesis, Genetic Humans Inhalation Exposure/adverse effects Long Interspersed Nucleotide Elements/drug effects Male Middle Aged Particulate Matter/adverse effects Prospective Studies Seasons Urban Population Vehicle Emissions/toxicity
Chemicals
Particulate Matter Vehicle Emissions
Authors & Affiliations
10 authors, click to expand affiliations / ORCID
Baccarelli Andrea
Laboratory of Environmental Epigenetics, Department of Environmental and Occupational Health, University of Milan, Via San Barnaba 8, 20122 Milan, Italy. [email protected]
Wright Robert O
Bollati Valentina
Tarantini Letizia
Litonjua Augusto A
Suh Helen H
Zanobetti Antonella
Sparrow David
Vokonas Pantel S
Schwartz Joel
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Article Info
Journal
American journal of respiratory and critical care medicine
Abbr.
Am J Respir Crit Care Med
ISSN
1535-4970
DOI
10.1164/rccm.200807-1097OC
Email
[email protected]
Published
2009-04-01
Epub
2009-00-08
Pages
572-8
Language
English
Region
United States
NLM ID
9421642
PMCID
PMC2720123
Subset
IM
Grants
NIEHS NIH HHS · ES00002 · United States
NIEHS NIH HHS · ES015172-01 · United States
NIEHS NIH HHS · P01 ES009825 · United States
Corrections
CommentIn
PMID: 19318540
CommentIn
PMID: 19897775
External Links
PubMed source DOI: 10.1164/rccm.200807-1097OC PMC Full Text View journal details
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