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PMID: 19136959 Published · ppublish English Journal Article Research Support, N.I.H., Extramural Research Support, Non-U.S. Gov't

High-resolution dynamic mapping of histone-DNA interactions in a nucleosome.

Nature structural & molecular biology ·Vol. 16 ·No. 2 ·2009-02-00 ·Pages 124-9

Hall MA, Shundrovsky A, Bai L, Fulbright RM, Lis JT, Wang MD

Abstract

The nature of the nucleosomal barrier that regulates access to the underlying DNA during many cellular processes is not fully understood. Here we present a detailed map of histone-DNA interactions along the DNA sequence to near base pair accuracy by mechanically unzipping single molecules of DNA, each containing a single nucleosome. This interaction map revealed a distinct approximately 5-bp periodicity that was enveloped by three broad regions of strong interactions, with the strongest occurring at the dyad and the other two about +/-40-bp from the dyad. Unzipping up to the dyad allowed recovery of a canonical nucleosome upon relaxation of the DNA, but unzipping beyond the dyad resulted in removal of the histone octamer from its initial DNA sequence. These findings have important implications for how RNA polymerase and other DNA-based enzymes may gain access to DNA associated with a nucleosome.

MeSH Terms
DNA/chemistry,metabolism DNA-Directed RNA Polymerases/metabolism HeLa Cells Histones/chemistry,metabolism Humans Nucleosomes/chemistry,metabolism
Chemicals
Histones Nucleosomes DNA DNA-Directed RNA Polymerases
Authors & Affiliations
6 authors, click to expand affiliations / ORCID
Hall Michael A
Department of Physics-Laboratory of Atomic and Solid State Physics, Cornell University, Ithaca, New York 14853, USA.
Shundrovsky Alla
Bai Lu
Fulbright Robert M
Lis John T
Wang Michelle D
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Article Info
Journal
Nature structural & molecular biology
Abbr.
Nat Struct Mol Biol
ISSN
1545-9985
DOI
10.1038/nsmb.1526
Published
2009-02-00
Epub
2009-00-11
Pages
124-9
Language
English
Region
United States
NLM ID
101186374
PMCID
PMC2635915
Subset
IM
Grants
NIGMS NIH HHS · R37 GM025232-22 · United States
NIGMS NIH HHS · GM25232 · United States
NIGMS NIH HHS · R37 GM025232-24 · United States
Howard Hughes Medical Institute · United States
NIGMS NIH HHS · R01 GM059849-07 · United States
NIGMS NIH HHS · R37 GM025232 · United States
NIGMS NIH HHS · R37 GM025232-26 · United States
NIGMS NIH HHS · R37 GM025232-23 · United States
NIGMS NIH HHS · R01 GM025232-31 · United States
NIGMS NIH HHS · R01 GM025232 · United States
NIGMS NIH HHS · R01 GM059849-06A1 · United States
NIGMS NIH HHS · R01 GM059849-08 · United States
NIGMS NIH HHS · R01 GM025232-28S1 · United States
NIGMS NIH HHS · R37 GM025232-25 · United States
NIGMS NIH HHS · R01 GM059849 · United States
NIGMS NIH HHS · R01 GM025232-28 · United States
NIGMS NIH HHS · R01 GM025232-30 · United States
NIGMS NIH HHS · R37 GM025232-27 · United States
NIGMS NIH HHS · R01 GM059849-09 · United States
NIGMS NIH HHS · GM059849 · United States
NIGMS NIH HHS · R01 GM025232-29 · United States
NIGMS NIH HHS · R37 GM025232-26S1 · United States
External Links
PubMed source DOI: 10.1038/nsmb.1526 PMC Full Text View journal details
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