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PMID: 19147696 Published · ppublish English Letter Research Support, Non-U.S. Gov't

Lin28 modulates cell growth and associates with a subset of cell cycle regulator mRNAs in mouse embryonic stem cells.

RNA (New York, N.Y.) ·Vol. 15 ·No. 3 ·2009-03-00 ·Pages 357-61

Xu B, Zhang K, Huang Y

Abstract

Lin28 is highly expressed in human and mouse embryonic stem (ES) cells. Here, we show that in mouse ES cells, specific repression of Lin28 results in decreased cell proliferation, while overexpression of Lin28 accelerates cell proliferation. Further, Lin28 associates specifically with ribonucleoprotein particles containing mRNAs for cyclins A and B and cdk4. Importantly, changes in Lin28 levels lead to corresponding changes in the levels of these proteins, and sequences from the 3' untranslated regions of cyclin B and cdk4 mRNAs exhibit stimulatory effects on translation of reporter genes in a Lin28-dependent fashion. Thus, we postulate that Lin28 may play a role in the regulation of translation of genes important for the growth and maintenance of pluripotent cells.

MeSH Terms
Animals Cell Cycle Cell Line Embryonic Stem Cells/metabolism Mice RNA, Messenger/metabolism RNA, Small Interfering/metabolism RNA-Binding Proteins/genetics,metabolism
Chemicals
Lin-28 protein, mouse RNA, Messenger RNA, Small Interfering RNA-Binding Proteins
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Xu Bingsen
Zhang Kexiong
Huang Yingqun
References (19)
19 references, click to expand
  1. The let-7 family of microRNAs.
    Trends Cell Biol. 2008 Oct;18(10):505-16 PMID: 18774294
  2. Selective blockade of microRNA processing by Lin28.
    Science. 2008 Apr 4;320(5872):97-100 PMID: 18292307
  3. Localization of the developmental timing regulator Lin28 to mRNP complexes, P-bodies and stress granules.
    RNA Biol. 2007 Jan-Mar;4(1):16-25 PMID: 17617744
  4. A feedback loop comprising lin-28 and let-7 controls pre-let-7 maturation during neural stem-cell commitment.
    Nat Cell Biol. 2008 Aug;10(8):987-93 PMID: 18604195
  5. The transcriptome profile of human embryonic stem cells as defined by SAGE.
    Stem Cells. 2004;22(1):51-64 PMID: 14688391
  6. AU-rich-element-mediated upregulation of translation by FXR1 and Argonaute 2.
    Cell. 2007 Mar 23;128(6):1105-18 PMID: 17382880
  7. The cold shock domain protein LIN-28 controls developmental timing in C. elegans and is regulated by the lin-4 RNA.
    Cell. 1997 Mar 7;88(5):637-46 PMID: 9054503
  8. Determinants of microRNA processing inhibition by the developmentally regulated RNA-binding protein Lin28.
    J Biol Chem. 2008 Aug 1;283(31):21310-4 PMID: 18550544
  9. Lin28 mediates the terminal uridylation of let-7 precursor MicroRNA.
    Mol Cell. 2008 Oct 24;32(2):276-84 PMID: 18951094
  10. The let-7 microRNA represses cell proliferation pathways in human cells.
    Cancer Res. 2007 Aug 15;67(16):7713-22 PMID: 17699775
  11. Cell cycle and developmental regulations of replication factors in mouse embryonic stem cells.
    J Biol Chem. 2005 Apr 1;280(13):12976-87 PMID: 15659392
  12. Temporally regulated expression of Lin-28 in diverse tissues of the developing mouse.
    Gene Expr Patterns. 2003 Dec;3(6):719-26 PMID: 14643679
  13. Cell-specific responses to loss of cyclin-dependent kinases.
    Oncogene. 2007 Jul 5;26(31):4469-77 PMID: 17297466
  14. Cell cycle control of embryonic stem cells.
    Stem Cell Rev. 2005;1(2):131-8 PMID: 17142847
  15. Induced pluripotent stem cell lines derived from human somatic cells.
    Science. 2007 Dec 21;318(5858):1917-20 PMID: 18029452
  16. Lin-28 binds IGF-2 mRNA and participates in skeletal myogenesis by increasing translation efficiency.
    Genes Dev. 2007 May 1;21(9):1125-38 PMID: 17473174
  17. Method to enhance transfection efficiency of cell lines and placental fibroblasts.
    Placenta. 2007 Aug-Sep;28(8-9):779-82 PMID: 17355893
  18. Lin-28 interaction with the Let-7 precursor loop mediates regulated microRNA processing.
    RNA. 2008 Aug;14(8):1539-49 PMID: 18566191
  19. Self-renewal of human embryonic stem cells is supported by a shortened G1 cell cycle phase.
    J Cell Physiol. 2006 Dec;209(3):883-93 PMID: 16972248
Article Info
Journal
RNA (New York, N.Y.)
Abbr.
RNA
ISSN
1469-9001
DOI
10.1261/rna.1368009
Published
2009-03-00
Epub
2009-00-15
Pages
357-61
Language
English
Region
United States
NLM ID
9509184
PMCID
PMC2657009
Subset
IM
External Links
PubMed source DOI: 10.1261/rna.1368009 PMC Full Text View journal details
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