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PMID: 1915284 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S.

The 3 A crystal structure of yeast initiator tRNA: functional implications in initiator/elongator discrimination.

The EMBO journal ·Vol. 10 ·No. 10 ·1991-10-00 ·Pages 3105-11

Basavappa R, Sigler PB

Abstract

A significantly improved molecular model of yeast initiator tRNA (ytRNA(iMet) has been prepared that gives insight into the structural basis of eukaryotic initiator tRNA's unique function. This study was made possible by X-ray data collected at synchrotron radiation sources with the newly developed technologies of 'imaging plates' and 'storage phosphors'. These data extend beyond the resolution limit of 4.0 A reported previously to a current limit of 3.0 A and are considerably more accurate. Refinement of the model against the new data (R factor = 21.5%) clearly reveals a novel modification and a set of tertiary interactions involving sequence features characteristic of eukaryotic initiator tRNAs. We hypothesize these to be the structural elements responsible for part of the special function of yeast tRNA(iMET).

MeSH Terms
Computer Simulation Models, Molecular Nucleic Acid Conformation Peptide Chain Initiation, Translational RNA, Fungal/chemistry RNA, Transfer/chemistry RNA, Transfer, Met/chemistry Stereoisomerism X-Ray Diffraction Yeasts/ultrastructure
Chemicals
RNA, Fungal RNA, Transfer, Met RNA, Transfer
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Basavappa R
Department of Biochemistry and Molecular Biology, University of Chicago.
Sigler P B
References (22)
22 references, click to expand
  1. Changing the acceptor identity of a transfer RNA by altering nucleotides in a "variable pocket".
    Science. 1988 Sep 30;241(4874):1804-7 PMID: 2459773
  2. Yeast tRNAAsp tertiary structure in solution and areas of interaction of the tRNA with aspartyl-tRNA synthetase. A comparative study of the yeast phenylalanine system by phosphate alkylation experiments with ethylnitrosourea.
    J Mol Biol. 1985 Aug 5;184(3):455-71 PMID: 3900415
  3. Presence of phosphorylated O-ribosyl-adenosine in T-psi-stem of yeast methionine initiator tRNA.
    Nucleic Acids Res. 1989 Feb 11;17(3):865-82 PMID: 2646591
  4. Escherichia coli formylmethionine tRNA: mutations in GGGCCC sequence conserved in anticodon stem of initiator tRNAs affect initiation of protein synthesis and conformation of anticodon loop.
    Proc Natl Acad Sci U S A. 1987 Jan;84(2):334-8 PMID: 3540960
  5. Crystallographic refinement of yeast aspartic acid transfer RNA.
    J Mol Biol. 1985 Jul 5;184(1):119-45 PMID: 3897553
  6. The role of modified purine 64 in initiator/elongator discrimination of tRNA(iMet) from yeast and wheat germ.
    Nucleic Acids Res. 1990 Aug 25;18(16):4677-82 PMID: 2395634
  7. Isoleucyl initiator tRNA does not initiate eucaryotic protein synthesis.
    J Biol Chem. 1984 Apr 25;259(8):4706-9 PMID: 6370992
  8. Three-dimensional structure of Escherichia coli initiator tRNAfMet.
    Nature. 1980 Jul 24;286(5771):346-51 PMID: 6157105
  9. Isolation and characterization of two methionine: tRNA ligases from wheat germ.
    Eur J Biochem. 1977 Aug 15;78(1):141-51 PMID: 913394
  10. Initiator tRNAs have a unique anticodon loop conformation.
    Proc Natl Acad Sci U S A. 1979 Jul;76(7):3289-93 PMID: 386336
  11. Crystal structure of a eukaryotic initiator tRNA.
    Nature. 1979 Mar 8;278(5700):188-90 PMID: 368656
  12. Further refinement of the structure of yeast tRNAPhe.
    J Mol Biol. 1978 Sep 25;124(3):523-34 PMID: 361973
  13. Crystal structure of yeast phenylalanine transfer RNA. I. Crystallographic refinement.
    J Mol Biol. 1978 Aug 25;123(4):607-30 PMID: 357742
  14. A crystallographic study of metal-binding to yeast phenylalanine transfer RNA.
    J Mol Biol. 1977 Apr 15;111(3):315-28 PMID: 325214
  15. Initiation of protein synthesis by folate-sufficient and folate-deficient Streptococcus faecalis R. Biochemical and biophysical properties of methionine transfer ribonucleic acid.
    J Biol Chem. 1974 Feb 25;249(4):1198-206 PMID: 4205317
  16. An analysis of the structure of tRNA.
    Annu Rev Biophys Bioeng. 1975;4(00):477-527 PMID: 1098566
  17. Transfer RNA: molecular structure, sequence, and properties.
    Annu Rev Biochem. 1976;45:805-60 PMID: 60910
  18. Structure of E. coli glutaminyl-tRNA synthetase complexed with tRNA(Gln) and ATP at 2.8 A resolution.
    Science. 1989 Dec 1;246(4934):1135-42 PMID: 2479982
  19. Structural features that underlie the use of bacterial Met-tRNAfMet primarily as an elongator in eukaryotic protein synthesis.
    J Biol Chem. 1989 Nov 5;264(31):18506-11 PMID: 2478546
  20. Structure of yeast phenylalanine transfer RNA at 2.5 A resolution.
    Proc Natl Acad Sci U S A. 1975 Nov;72(11):4414-8 PMID: 1105583
  21. Biochemical and physical characterization of an unmodified yeast phenylalanine transfer RNA transcribed in vitro.
    Proc Natl Acad Sci U S A. 1988 Feb;85(4):1033-7 PMID: 3277187
  22. Changing the identity of a tRNA by introducing a G-U wobble pair near the 3' acceptor end.
    Science. 1988 May 6;240(4853):793-6 PMID: 2452483
Article Info
Journal
The EMBO journal
Abbr.
EMBO J
ISSN
0261-4189
Published
1991-10-00
Pages
3105-11
Language
English
Region
England
NLM ID
8208664
PMCID
PMC453028
Subset
IM
Grants
NIGMS NIH HHS · GM 07183 · United States
NIGMS NIH HHS · GM 15225 · United States
External Links
PubMed source PMC Full Text View journal details
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