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PMID: 19156129 Published · ppublish English Journal Article Research Support, N.I.H., Extramural Research Support, Non-U.S. Gov't

An integrated workflow for charting the human interaction proteome: insights into the PP2A system.

Molecular systems biology ·Vol. 5 ·2009-00-00 ·Pages 237

Glatter T, Wepf A, Aebersold R, Gstaiger M

Abstract

Protein complexes represent major functional units for the execution of biological processes. Systematic affinity purification coupled with mass spectrometry (AP-MS) yielded a wealth of information on the compendium of protein complexes expressed in Saccharomyces cerevisiae. However, global AP-MS analysis of human protein complexes is hampered by the low throughput, sensitivity and data robustness of existing procedures, which limit its application for systems biology research. Here, we address these limitations by a novel integrated method, which we applied and benchmarked for the human protein phosphatase 2A system. We identified a total of 197 protein interactions with high reproducibility, showing the coexistence of distinct classes of phosphatase complexes that are linked to proteins implicated in mitosis, cell signalling, DNA damage control and more. These results show that the presented analytical process will substantially advance throughput and reproducibility in future systematic AP-MS studies on human protein complexes.

MeSH Terms
Affinity Labels Cell Line Chromatography, Affinity Humans Protein Phosphatase 2/genetics,metabolism Proteome Saccharomyces cerevisiae/genetics Sensitivity and Specificity Tandem Mass Spectrometry
Chemicals
Affinity Labels Proteome Protein Phosphatase 2
Authors & Affiliations
4 authors, click to expand affiliations / ORCID
Glatter Timo
Institute of Molecular Systems Biology, ETH Zurich, Zurich, Switzerland.
Wepf Alexander
Aebersold Ruedi
Gstaiger Matthias
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Article Info
Journal
Molecular systems biology
Abbr.
Mol Syst Biol
ISSN
1744-4292
DOI
10.1038/msb.2008.75
Published
2009-00-00
Epub
2009-00-20
Pages
237
Language
English
Region
England
NLM ID
101235389
PMCID
PMC2644174
Subset
IM
Grants
NHLBI NIH HHS · N01HV28179 · United States
NHLBI NIH HHS · N01-HV-28179 · United States
External Links
PubMed source DOI: 10.1038/msb.2008.75 PMC Full Text View journal details
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