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PMID: 1916233 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Lack of specific hybridization between the lep genes of Salmonella typhimurium and Bacillus licheniformis.

FEMS microbiology letters ·Vol. 65 ·No. 3 ·1991-07-01 ·Pages 345-51

van Dijl JM, de Jong A, Smith H, Bron S, Venema G

Abstract

This paper describes an attempt to clone the Bacillus licheniformis lep gene, encoding signal peptidase, using the Salmonella typhimurium lep gene as a hybridization probe. Although a hybridizing fragment was obtained, DNA sequence analysis indicated that it did not contain the lep gene. Instead, the protein encoded by the cloned fragment showed similarity with a variety of L-asparaginases.

MeSH Terms

Amino Acid Sequence Asparaginase/genetics Bacillus/genetics Bacteriophages/genetics Base Sequence Blotting, Southern Cloning, Molecular DNA Probes/genetics Endopeptidases/genetics Escherichia coli/genetics Membrane Proteins Molecular Sequence Data Restriction Mapping Salmonella typhimurium/genetics Serine Endopeptidases

Chemicals

DNA Probes Membrane Proteins Endopeptidases Serine Endopeptidases type I signal peptidase Asparaginase

Authors & Affiliations

5 authors, click to expand affiliations / ORCID

van Dijl J M

Department of Genetics, Center of Biological Sciences, Haren, The Netherlands.

de Jong A

Smith H

Bron S

Venema G

Article Info

Journal

FEMS microbiology letters

Abbr.

FEMS Microbiol Lett

ISSN

0378-1097

DOI

10.1016/0378-1097(91)90239-7

Published

1991-07-01

Pages

345-51

Language

English

Region

England

NLM ID

7705721

Subset

Databases

GENBANK

S59339, S70107, S70109, S70115, S70117, S70121, S70125, S70128, S70130, S76128

External Links

PubMed source DOI: 10.1016/0378-1097(91)90239-7 View journal details

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Lack of specific hybridization between the lep genes of Salmonella typhimurium and Bacillus licheniformis.

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