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PMID: 191638 Published · ppublish English Journal Article Research Support, U.S. Gov't, P.H.S.

Immunological reactivity of antisera to sodium dodecyl sulfate-derived polypeptides of polyoma virions.

Journal of virology ·Vol. 21 ·No. 3 ·1977-03-00 ·Pages 1113-20

McMillen J, Consigli RA

Abstract

A study was undertaken to produce antisera to sodium dodecyl sulfate-derived polyoma virion polypeptides. With the use of this antisera, it was possible to detect, by immunofluorescence, cytoplasmic synthesis of V1, V2, and V3 polypeptides at 18 h postinfection and subsequent transport to the nucleus by 22 h postinfection. Anti-V1, anti-V2, and anti-V3 sera did not react with intact virions in an immunodiffusion assay, nor did they possess hemagglutination inhibition or viral neutralization activity. Antiserum produced against the four host histone polypeptides (V4 through V7) demonstrated immunofluorescence when reacted with polyoma-infected cells but not with uninfected cells. Antihistone serum was also capable of neutralizing viral infectivity, inhibiting hemagglutination and reacting with whole virions in an immunodiffusion assay.

MeSH Terms
Animals Antigens, Viral Cell Line Cell Nucleus/metabolism Cytoplasm/metabolism Electrophoresis, Polyacrylamide Gel Fluorescent Antibody Technique Hemagglutination Inhibition Tests Immune Sera Immunoglobulin Fab Fragments Immunoglobulin G Neutralization Tests Peptide Biosynthesis Peptides/immunology Polyomavirus/immunology,metabolism Rabbits/immunology Sodium Dodecyl Sulfate Viral Proteins/biosynthesis,immunology
Chemicals
Antigens, Viral Immune Sera Immunoglobulin Fab Fragments Immunoglobulin G Peptides Viral Proteins Sodium Dodecyl Sulfate
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
McMillen J
Consigli R A
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25 references, click to expand
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Article Info
Journal
Journal of virology
Abbr.
J Virol
ISSN
0022-538X
DOI
10.1128/JVI.21.3.1113-1120.1977
Published
1977-03-00
Pages
1113-20
Language
English
Region
United States
NLM ID
0113724
PMCID
PMC515652
Subset
IM
External Links
PubMed source DOI: 10.1128/JVI.21.3.1113-1120.1977 PMC Full Text View journal details
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