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PMID: 19171755 Published · ppublish English Journal Article Research Support, N.I.H., Extramural Research Support, U.S. Gov't, Non-P.H.S.

TREM-2 (triggering receptor expressed on myeloid cells 2) is a phagocytic receptor for bacteria.

The Journal of cell biology ·Vol. 184 ·No. 2 ·2009-01-26 ·Pages 215-23

N'Diaye EN, Branda CS, Branda SS, Nevarez L, Colonna M, Lowell C, Hamerman JA, Seaman WE

Abstract

Phagocytosis, which is essential for the immune response to pathogens, is initiated by specific interactions between pathogens and cell surface receptors expressed by phagocytes. This study identifies triggering receptor expressed on myeloid cells 2 (TREM-2) and its signaling counterpart DAP12 as a molecular complex that promotes phagocytosis of bacteria. Expression of TREM-2-DAP12 enables nonphagocytic Chinese hamster ovary cells to internalize bacteria. This function depends on actin cytoskeleton dynamics and the activity of the small guanosine triphosphatases Rac and Cdc42. Internalization also requires src kinase activity and tyrosine phosphorylation. In bone marrow-derived macrophages, phagocytosis is decreased in the absence of DAP12 and can be restored by expression of TREM-2-DAP12. Depletion of TREM-2 inhibits both binding and uptake of bacteria. Finally, TREM-2-dependent phagocytosis is impaired in Syk-deficient macrophages. This study highlights a novel role for TREM-2-DAP12 in the immune response to bacterial pathogens.

MeSH Terms
Adaptor Proteins, Signal Transducing/metabolism Animals Bacteria/immunology,metabolism Bacterial Physiological Phenomena CHO Cells Cells, Cultured Cricetinae Cricetulus Escherichia coli/metabolism Membrane Glycoproteins/metabolism Mice Mice, Knockout Myeloid Cells/immunology,metabolism Phagocytosis/immunology Receptors, Immunologic/metabolism Staphylococcus aureus/metabolism
Chemicals
Adaptor Proteins, Signal Transducing Membrane Glycoproteins Receptors, Immunologic Trem2 protein, mouse Tyrobp protein, mouse
Authors & Affiliations
8 authors, click to expand affiliations / ORCID
N'Diaye Elsa-Noah
Macrophage Biology Laboratory, San Francisco VA Medical Center, San Francisco, CA 94121, USA.
Branda Catherine S
Branda Steven S
Nevarez Lisette
Colonna Marco
Lowell Clifford
Hamerman Jessica A
Seaman William E
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Article Info
Journal
The Journal of cell biology
Abbr.
J Cell Biol
ISSN
1540-8140
DOI
10.1083/jcb.200808080
Published
2009-01-26
Pages
215-23
Language
English
Region
United States
NLM ID
0375356
PMCID
PMC2654305
Subset
IM
Grants
NIGMS NIH HHS · GM 062114 · United States
NIGMS NIH HHS · U54 GM062114 · United States
NCI NIH HHS · R01 CA 87922 · United States
NCI NIH HHS · R01 CA087922 · United States
NIAID NIH HHS · R01 AI073441 · United States
External Links
PubMed source DOI: 10.1083/jcb.200808080 PMC Full Text View journal details
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