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PMID: 191830 Published · ppublish English Journal Article Research Support, U.S. Gov't, P.H.S.

Tumorigenicity of simian virus 40-transformed human cells and mouse--human hybrids in nude mice.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 74 ·No. 3 ·1977-03-00 ·Pages 1142-6

Koprowski H, Croce CM

Abstract

Four different human cell lines transformed by simian virus 40 (SV40) were tested for their tumorigenicity in athymic nude mice. Two of these lines, W18Va2 and GM52VA, were found to be tumorigenic when inoculated at a concentration of 1 x 10(7) cells per mouse. The other two cell lines, LN-SV and GM54VA, were found to induce very small tumors only after the injection of approximately 1 x 10(8) cells per mouse. Somatic cell hybrids between either LN-SV or GM54VA SV40-transformed human cells and normal mouse peritoneal macrophages, which have retained the human chromosomes carrying the SV40 genome, were found to be much more tumorigenic than the SV40-transformed human cell parents. These experiments suggest that the genetic background in which the human chromosomes carrying the SV40 genome are present plays a role in the modulation of the expiration of malignancy.

MeSH Terms
Animals Antigens, Viral/analysis Cell Line Cell Transformation, Neoplastic/pathology Genes Humans Hybrid Cells/physiology Karyotyping Macrophages Mice Mice, Nude Neoplasms, Experimental/genetics,pathology Simian virus 40/immunology
Chemicals
Antigens, Viral
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Koprowski H
Croce C M
References (17)
17 references, click to expand
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Article Info
Journal
Proceedings of the National Academy of Sciences of the United States of America
Abbr.
Proc Natl Acad Sci U S A
ISSN
0027-8424
DOI
10.1073/pnas.74.3.1142
Published
1977-03-00
Pages
1142-6
Language
English
Region
United States
NLM ID
7505876
PMCID
PMC430625
Subset
IM
External Links
PubMed source DOI: 10.1073/pnas.74.3.1142 PMC Full Text View journal details
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