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PMID: 19211702 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Exploring the mechanism of Physcomitrella patens desiccation tolerance through a proteomic strategy.

Plant physiology ·Vol. 149 ·No. 4 ·2009-04-00 ·Pages 1739-50

Wang XQ, Yang PF, Liu Z, Liu WZ, Hu Y, Chen H, Kuang TY, Pei ZM, Shen SH, He YK

Abstract

The moss Physcomitrella patens has been shown to tolerate abiotic stresses, including salinity, cold, and desiccation. To better understand this plant's mechanism of desiccation tolerance, we have applied cellular and proteomic analyses. Gametophores were desiccated over 1 month to 10% of their original fresh weight. We report that during the course of dehydration, several related processes are set in motion: plasmolysis, chloroplast remodeling, and microtubule depolymerization. Despite the severe desiccation, the membrane system maintains integrity. Through two-dimensional gel electrophoresis and image analysis, we identified 71 proteins as desiccation responsive. Following identification and functional categorization, we found that a majority of the desiccation-responsive proteins were involved in metabolism, cytoskeleton, defense, and signaling. Degradation of cytoskeletal proteins might result in cytoskeletal disassembly and consequent changes in the cell structure. Late embryogenesis abundant proteins and reactive oxygen species-scavenging enzymes are both prominently induced, and they might help to diminish the damage brought by desiccation.

MeSH Terms
Adaptation, Physiological Bryopsida/cytology,immunology,physiology,ultrastructure Chlorophyll/metabolism Chromatography, Liquid Cytoskeletal Proteins/metabolism Desiccation Electrophoresis, Gel, Two-Dimensional Microtubules/metabolism,ultrastructure Plant Proteins/metabolism Proteome/metabolism Proteomics/methods Signal Transduction Stress, Physiological Tandem Mass Spectrometry
Chemicals
Cytoskeletal Proteins Plant Proteins Proteome Chlorophyll
Authors & Affiliations
10 authors, click to expand affiliations / ORCID
Wang Xiao Qin
College of Life Sciences, Capital Normal University, Beijing 100048, China.
Yang Ping Fang
Liu Zheng
Liu Wei Zhong
Hu Yong
Chen Hui
Kuang Ting Yun
Pei Zhen Ming
Shen Shi Hua
He Yi Kun
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Article Info
Journal
Plant physiology
Abbr.
Plant Physiol
ISSN
0032-0889
DOI
10.1104/pp.108.131714
Published
2009-04-00
Epub
2009-00-11
Pages
1739-50
Language
English
Region
United States
NLM ID
0401224
PMCID
PMC2663739
Subset
IM
Corrections
CommentIn
PMID: 19816114
External Links
PubMed source DOI: 10.1104/pp.108.131714 PMC Full Text View journal details
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