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PMID: 19217374 Published · ppublish English Journal Article Research Support, N.I.H., Extramural Research Support, Non-U.S. Gov't

FARP1 promotes the dendritic growth of spinal motor neuron subtypes through transmembrane Semaphorin6A and PlexinA4 signaling.

Neuron ·Vol. 61 ·No. 3 ·2009-02-12 ·Pages 359-72

Zhuang B, Su YS, Sockanathan S

Abstract

The dendritic morphology of neurons dictates their abilities to process and transmit information; however, the signaling pathways that regulate dendritic growth and complexity are poorly understood. Here, we show that retinoids induce the expression of the FERM Rho-GEF protein FARP1 in the developing spinal cord. FARP1 is expressed in subsets of motor neurons and is enriched in dendrites of lateral motor column (LMC) neurons that innervate the limb. FARP1 is necessary and sufficient to promote LMC dendritic growth but does not affect dendrite number or axonal morphology. We show that FARP1 serves as a specific effector of transmembrane Semaphorin6A and PlexinA4 signals to regulate LMC dendritic growth, and that its Rho-GEF domain is necessary for this function. These findings reveal that retinoid and Sema6A/PlexA4 signaling pathways intersect through FARP1 to control dendritic growth, and uncover the existence of subtype-specific signaling networks that control dendritic developmental programs in spinal motor neurons.

MeSH Terms
Animals Avian Proteins/genetics,isolation & purification,metabolism Cell Differentiation/drug effects,physiology Cell Membrane/metabolism,ultrastructure Chick Embryo Cytoskeletal Proteins/genetics,metabolism Dendrites/drug effects,metabolism,ultrastructure Gene Expression Regulation, Developmental/drug effects,physiology Guanine Nucleotide Exchange Factors/genetics,metabolism Humans Motor Neurons/cytology,drug effects,metabolism Neurogenesis/drug effects,physiology Protein Structure, Tertiary/drug effects,physiology Receptors, Cell Surface/genetics,metabolism Retinoids/metabolism,pharmacology Rho Guanine Nucleotide Exchange Factors Semaphorins/genetics,metabolism Signal Transduction/drug effects,genetics Spinal Cord/cytology,metabolism rho GTP-Binding Proteins/genetics,metabolism
Chemicals
Avian Proteins Cytoskeletal Proteins FARP1 protein, human Guanine Nucleotide Exchange Factors Plxna4 protein, human Receptors, Cell Surface Retinoids Rho Guanine Nucleotide Exchange Factors SEMA6A protein, human Semaphorins rho GTP-Binding Proteins
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Zhuang BinQuan
The Solomon Snyder Department of Neuroscience, The Johns Hopkins University School of Medicine, 725 N. Wolfe Street, Baltimore, MD 21205, USA.
Su YouRong Sophie
Sockanathan Shanthini
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Article Info
Journal
Neuron
Abbr.
Neuron
ISSN
1097-4199
DOI
10.1016/j.neuron.2008.12.022
Published
2009-02-12
Pages
359-72
Language
English
Region
United States
NLM ID
8809320
PMCID
PMC2654783
Subset
IM
Grants
NINDS NIH HHS · R01 NS046336-02 · United States
NINDS NIH HHS · R01 NS046336-01 · United States
NINDS NIH HHS · R01 NS046336-04 · United States
NINDS NIH HHS · R01 NS046336 · United States
NINDS NIH HHS · R01 NS046336-03 · United States
External Links
PubMed source DOI: 10.1016/j.neuron.2008.12.022 PMC Full Text View journal details
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