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PMID: 1923764 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, Non-P.H.S. Research Support, U.S. Gov't, P.H.S.

Effects of an abasic site on triple helix formation characterized by affinity cleaving.

Nucleic acids research ·Vol. 19 ·No. 18 ·1991-09-25 ·Pages 4963-5

Horne DA, Dervan PB

Abstract

The stability of triple helical complexes of pyrimidine oligodeoxyribonucleotides containing one abasic 1,2-dideoxy-D-ribose (phi) residue was examined by affinity cleaving. Within a pyrimidine third strand, the triplets phi.AT, phi.GC, phi.TA and phi.CG are significantly less stable than the triplets, T.AT, C+GC and G.TA. The decrease in binding produced by an abasic residue is similar to that observed with imperfectly matched natural base triplets, with phi.AT and phi.GC being less stable than phi.TA and phi.CG triplets for the sequences studied.

MeSH Terms
Base Composition Base Sequence Binding Sites Deoxyribose/analogs & derivatives Edetic Acid Molecular Sequence Data Nucleic Acid Conformation Oligodeoxyribonucleotides/chemistry Pyrimidines/chemistry
Chemicals
Oligodeoxyribonucleotides Pyrimidines Deoxyribose 1,2-dideoxyribose Edetic Acid
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Horne D A
Arnold and Mabel Beckman Laboratory of Chemical Synthesis, California Institute of Technology, Pasadena 91125.
Dervan P B
References (13)
13 references, click to expand
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Article Info
Journal
Nucleic acids research
Abbr.
Nucleic Acids Res
ISSN
0305-1048
DOI
10.1093/nar/19.18.4963
Published
1991-09-25
Pages
4963-5
Language
English
Region
England
NLM ID
0411011
PMCID
PMC328797
Subset
IM
External Links
PubMed source DOI: 10.1093/nar/19.18.4963 PMC Full Text View journal details
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