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PMID: 1924365 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S.

Reversal of synaptic depression by serotonin at Aplysia sensory neuron synapses involves activation of adenylyl cyclase.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 88 ·No. 20 ·1991-10-15 ·Pages 9021-5

Goldsmith BA, Abrams TW

Abstract

Facilitation of the monosynaptic connection between siphon sensory neurons and gill and siphon motor neuron contributes to sensitization and dishabituation of the gill and siphon withdrawal reflex in Aplysia. The facilitatory transmitter serotonin (5-HT) initiates two mechanisms that act in parallel to increase transmitter release from siphon sensory neurons. 5-HT acts, at least partly through cAMP, to broaden the presynaptic action potential. 5-HT also initiates a second process that facilitates depressed sensory neuron synapses by a mechanism independent of changes in action potential duration. Recent experiments indicated that either of two protein kinases, cAMP-dependent protein kinase A and protein kinase C, are capable of effectively activating this second facilitatory mechanism, restoring synaptic transmission in depressed synapses. We have used the adenylyl cyclase inhibitor SQ 22,536 [9-(tetrahydro-2-furyl)adenine or THFA] to explore the contribution of cAMP to the reversal of synaptic depression. THFA effectively inhibited both adenylyl cyclase activity in vitro and known cyclase-mediated effects in intact sensory neurons. THFA also completely blocked facilitation of depressed synapses by 5-HT. These results suggest that adenylyl cyclase plays a critical role in the reversal of synaptic depression that contributes to dishabituation in this system.

MeSH Terms
Adenine/analogs & derivatives,pharmacology Adenylyl Cyclase Inhibitors Adenylyl Cyclases/metabolism Animals Aplysia Enzyme Activation Evoked Potentials/drug effects Ganglia/physiology In Vitro Techniques Kinetics Neurons, Afferent/drug effects,enzymology,physiology Phorbol 12,13-Dibutyrate/pharmacology Serotonin/pharmacology Synapses/drug effects,enzymology,physiology
Chemicals
Adenylyl Cyclase Inhibitors 9-(tetrahydro-2-furyl)-adenine Serotonin Phorbol 12,13-Dibutyrate Adenylyl Cyclases Adenine
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Goldsmith B A
Department of Biology, University of Pennsylvania, Philadelphia 19104.
Abrams T W
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38 references, click to expand
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Article Info
Journal
Proceedings of the National Academy of Sciences of the United States of America
Abbr.
Proc Natl Acad Sci U S A
ISSN
0027-8424
DOI
10.1073/pnas.88.20.9021
Published
1991-10-15
Pages
9021-5
Language
English
Region
United States
NLM ID
7505876
PMCID
PMC52643
Subset
IM
Grants
NINDS NIH HHS · NS 25788 · United States
External Links
PubMed source DOI: 10.1073/pnas.88.20.9021 PMC Full Text View journal details
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