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PMID: 19244141 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

The cytosolic protein response as a subcomponent of the wider heat shock response in Arabidopsis.

The Plant cell ·Vol. 21 ·No. 2 ·2009-02-00 ·Pages 642-54

Sugio A, Dreos R, Aparicio F, Maule AJ

Abstract

In common with a range of environmental and biological stresses, heat shock results in the accumulation of misfolded proteins and a collection of downstream consequences for cellular homeostasis and growth. Within this complex array of responses, the sensing of and responses to misfolded proteins in specific subcellular compartments involves specific chaperones, transcriptional regulators, and expression profiles. Using biological (ectopic protein expression and virus infection) and chemical triggers for misfolded protein accumulation, we have profiled the transcriptional features of the response to misfolded protein accumulation in the cytosol (i.e., the cytoplasmic protein response [CPR]) and identified the effects as a subcomponent of the wider effects induced by heat shock. The CPR in Arabidopsis thaliana is associated with the heat shock promoter element and the involvement of specific heat shock factors (HSFs), notably HSFA2, which appears to be regulated by alternative splicing and non-sense-mediated decay. Characterization of Arabidopsis HSFA2 knockout and overexpression lines showed that HSFA2 is one of the regulatory components of the CPR.

MeSH Terms
Alternative Splicing Arabidopsis/drug effects,metabolism,physiology Arabidopsis Proteins Azetidinecarboxylic Acid/pharmacology Cytosol/metabolism DNA-Binding Proteins/genetics,metabolism,physiology Gene Expression Profiling Heat Shock Transcription Factors Heat-Shock Proteins/genetics,metabolism,physiology Heat-Shock Response/physiology Plant Proteins/genetics,metabolism,physiology Promoter Regions, Genetic Protein Folding Transcription Factors/genetics,metabolism,physiology Transcription, Genetic Tunicamycin/pharmacology
Chemicals
Arabidopsis Proteins DNA-Binding Proteins HSFA2 protein, Arabidopsis Heat Shock Transcription Factors Heat-Shock Proteins Plant Proteins Transcription Factors Tunicamycin Azetidinecarboxylic Acid
Authors & Affiliations
4 authors, click to expand affiliations / ORCID
Sugio Akiko
John Ines Centre, Colney, Norwich NR4 7UH, United Kingdom.
Dreos René
Aparicio Frederic
Maule Andrew J
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Article Info
Journal
The Plant cell
Abbr.
Plant Cell
ISSN
1040-4651
DOI
10.1105/tpc.108.062596
Published
2009-02-00
Epub
2009-00-24
Pages
642-54
Language
English
Region
England
NLM ID
9208688
PMCID
PMC2660624
Subset
IM
Grants
Biotechnology and Biological Sciences Research Council · United Kingdom
External Links
PubMed source DOI: 10.1105/tpc.108.062596 PMC Full Text View journal details
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