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PMID: 192908 Published · ppublish English Journal Article

Solubilization of the Epstein-Barr virus-determined nuclear antigen and its characterization as a DNA-binding protein.

Journal of virology ·Vol. 22 ·No. 1 ·1977-04-00 ·Pages 1-8

Luka J, Siegert W, Klein G

Abstract

The Eptstein-Barr virus (EBV)-determined nuclear antigen (EBNA) was solubilized from isolate nuclei of two EBV-transformed cell lines- Raji and AW-Ramos, by high-salt treatment. Its DNA-binding properties were studied by DNA-cellulose chromatography and a 51Cr release complement fixation assay. EBNA binds to both double-stranded and single-stranded calf thymus DNA, showing a higher affinity to double-stranded DNA. There was no detectable difference in the DNA binding of EBNA prepared from Raji and AW-Ramos cells.

MeSH Terms
Antigens, Viral/isolation & purification Burkitt Lymphoma Cell Line Cell Nucleus/immunology Complement Fixation Tests DNA/metabolism Herpesvirus 4, Human/immunology,metabolism Humans Protein Binding Solubility Surface-Active Agents Urea Viral Proteins/immunology,isolation & purification,metabolism
Chemicals
Antigens, Viral Surface-Active Agents Viral Proteins Urea DNA
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Luka J
Siegert W
Klein G
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34 references, click to expand
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Article Info
Journal
Journal of virology
Abbr.
J Virol
ISSN
0022-538X
DOI
10.1128/JVI.22.1.1-8.1977
Published
1977-04-00
Pages
1-8
Language
English
Region
United States
NLM ID
0113724
PMCID
PMC515679
Subset
IM
External Links
PubMed source DOI: 10.1128/JVI.22.1.1-8.1977 PMC Full Text View journal details
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