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PMID: 19306879 Published · ppublish English Journal Article Research Support, N.I.H., Extramural

Focal adhesion disassembly requires clathrin-dependent endocytosis of integrins.

FEBS letters ·Vol. 583 ·No. 8 ·2009-04-17 ·Pages 1337-43

Chao WT, Kunz J

Abstract

Cell migration requires the controlled disassembly of focal adhesions, but the underlying mechanisms remain poorly understood. Here, we show that adhesion turnover is mediated through dynamin- and clathrin-dependent endocytosis of activated beta1 integrins. Consistent with this, clathrin and the clathrin adaptors AP-2 and disabled-2 (DAB2) distribute along with dynamin 2 to adhesion sites prior to adhesion disassembly. Moreover, knockdown of either dynamin 2 or both clathrin adaptors blocks beta1 integrin internalization, leading to impaired focal adhesion disassembly and cell migration. Together, these results provide important insight into the mechanisms underlying adhesion disassembly and identify novel components of the disassembly pathway.

MeSH Terms
Cell Line, Tumor Clathrin/physiology Endocytosis/physiology Fluorescent Antibody Technique Humans Integrins/physiology RNA, Small Interfering
Chemicals
Clathrin Integrins RNA, Small Interfering
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Chao Wei-Ting
Department of Molecular Physiology and Biophysics, Baylor College of Medicine, 1 Baylor Plaza, BCM335, RM T419, Houston, TX 77030, USA.
Kunz Jeannette
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Article Info
Journal
FEBS letters
Abbr.
FEBS Lett
ISSN
1873-3468
DOI
10.1016/j.febslet.2009.03.037
Published
2009-04-17
Epub
2009-00-22
Pages
1337-43
Language
English
Region
England
NLM ID
0155157
PMCID
PMC2801759
Subset
IM
Grants
NIGMS NIH HHS · R01 GM068098 · United States
NIGMS NIH HHS · R01 GM068098-05 · United States
NIGMS NIH HHS · GM068098 · United States
External Links
PubMed source DOI: 10.1016/j.febslet.2009.03.037 PMC Full Text View journal details
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