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PMID: 19328791 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Review

Glycoproteomics: past, present and future.

FEBS letters ·Vol. 583 ·No. 11 ·2009-06-05 ·Pages 1728-35

Tissot B, North SJ, Ceroni A, Pang PC, Panico M, Rosati F, Capone A, Haslam SM, Dell A, Morris HR

Abstract

This invited paper reviews the study of protein glycosylation, commonly known as glycoproteomics, beginning with the origins of the subject area in the early 1970s shortly after mass spectrometry was first applied to protein sequencing. We go on to describe current analytical approaches to glycoproteomic analyses, with exemplar projects presented in the form of the complex story of human glycodelin and the characterisation of blood group H eptitopes on the O-glycans of gp273 from Unio elongatulus. Finally, we present an update on the latest progress in the field of automated and semi-automated interpretation and annotation of these data in the form of GlycoWorkBench, a powerful informatics tool that provides valuable assistance in unravelling the complexities of glycoproteomic studies.

MeSH Terms
Carbohydrates/chemistry Mass Spectrometry Proteomics
Chemicals
Carbohydrates
Authors & Affiliations
10 authors, click to expand affiliations / ORCID
Tissot Bérangère
Division of Molecular Biosciences, Faculty of Natural Sciences, Imperial College London, London SW7 2AZ, United Kingdom.
North Simon J
Ceroni Alessio
Pang Poh-Choo
Panico Maria
Rosati Floriana
Capone Antonietta
Haslam Stuart M
Dell Anne
Morris Howard R
References (43)
43 references, click to expand
  1. Chip-mass spectrometry for glycomic studies.
    Mass Spectrom Rev. 2009 Mar-Apr;28(2):223-53 PMID: 19145581
  2. Neisseria gonorrhoeae type IV pili undergo multisite, hierarchical modifications with phosphoethanolamine and phosphocholine requiring an enzyme structurally related to lipopolysaccharide phosphoethanolamine transferases.
    J Biol Chem. 2006 Sep 22;281(38):27712-23 PMID: 16825186
  3. Efficacy of glycoprotein enrichment by microscale lectin affinity chromatography.
    J Sep Sci. 2008 Aug;31(14):2722-32 PMID: 18623281
  4. Effects of differential glycosylation of glycodelins on lymphocyte survival.
    J Biol Chem. 2009 May 29;284(22):15084-96 PMID: 19240032
  5. N-linked glycosylation in Campylobacter jejuni and its functional transfer into E. coli.
    Science. 2002 Nov 29;298(5599):1790-3 PMID: 12459590
  6. Differential analysis of site-specific glycans on plasma and cellular fibronectins: application of a hydrophilic affinity method for glycopeptide enrichment.
    Glycobiology. 2005 Dec;15(12):1332-40 PMID: 16037490
  7. Automated N-glycopeptide identification using a combination of single- and tandem-MS.
    J Proteome Res. 2007 Oct;6(10):3995-4005 PMID: 17727280
  8. Amino acid sequence and post-translational modification of human interleukin 2.
    Proc Natl Acad Sci U S A. 1984 Oct;81(20):6486-90 PMID: 6333684
  9. Determination of the sequences of protein-derived peptides and peptide mixtures by mass spectrometry.
    Biochem J. 1971 Nov;125(1):189-201 PMID: 5158904
  10. Automatic annotation of matrix-assisted laser desorption/ionization N-glycan spectra.
    Proteomics. 2005 Mar;5(4):865-75 PMID: 15693066
  11. Glycosylation analysis of glycoproteins and proteoglycans using capillary electrophoresis-mass spectrometry strategies.
    Electrophoresis. 2008 Jun;29(12):2485-507 PMID: 18512669
  12. A non-Golgi alpha 1,2-fucosyltransferase that modifies Skp1 in the cytoplasm of Dictyostelium.
    J Biol Chem. 2001 Sep 7;276(36):33952-63 PMID: 11423539
  13. Isolation and characterization of human calcitonin gene-related peptide.
    Nature. 1984 Apr 19-25;308(5961):746-8 PMID: 6609312
  14. Some problems associated with the amino acid-sequence analysis of proteins by mass spectrometry.
    Biochem J. 1969 Mar;111(5):38P PMID: 5783462
  15. Glycoprotein structure determination by mass spectrometry.
    Science. 2001 Mar 23;291(5512):2351-6 PMID: 11269315
  16. A novel sialylated N-acetylgalactosamine-containing oligosaccharide is the major complex-type structure present in Bowes melanoma tissue plasminogen activator.
    Glycobiology. 1991 Mar;1(2):173-85 PMID: 1823160
  17. Comparison of the methods for profiling glycoprotein glycans--HUPO Human Disease Glycomics/Proteome Initiative multi-institutional study.
    Glycobiology. 2007 Apr;17(4):411-22 PMID: 17223647
  18. Sialic acid capping of CD8beta core 1-O-glycans controls thymocyte-major histocompatibility complex class I interaction.
    J Biol Chem. 2003 Feb 28;278(9):7240-6 PMID: 12459555
  19. Flagellar glycosylation in Clostridium botulinum.
    FEBS J. 2008 Sep;275(17):4428-44 PMID: 18671733
  20. Mass spectrometric analysis of N- and O-glycosylation of tissues and cells.
    Curr Opin Struct Biol. 2006 Oct;16(5):584-91 PMID: 16938453
  21. Matrix-assisted laser desorption/ionization time-of-flight mass spectrometry and electron microscopy for the characterization of the vitelline coat glycoproteins of the polarized egg of Unio elongatulus.
    Mol Reprod Dev. 1997 Dec;48(4):511-7 PMID: 9364446
  22. GlycoWorkbench: a tool for the computer-assisted annotation of mass spectra of glycans.
    J Proteome Res. 2008 Apr;7(4):1650-9 PMID: 18311910
  23. The Lewis x epitope is a major non-reducing structure in the sulphated N-glycans attached to Asn-65 of bovine pro-opiomelanocortin.
    Glycobiology. 1993 Jun;3(3):225-39 PMID: 7689367
  24. The GlycanBuilder: a fast, intuitive and flexible software tool for building and displaying glycan structures.
    Source Code Biol Med. 2007 Aug 07;2:3 PMID: 17683623
  25. Glycan family analysis for deducing N-glycan topology from single MS.
    Bioinformatics. 2009 Feb 1;25(3):365-71 PMID: 19073587
  26. Mass spectrometry and the emerging field of glycomics.
    Chem Biol. 2008 Sep 22;15(9):881-92 PMID: 18804025
  27. Bacterial glycoproteomics.
    Microbiology (Reading). 2006 Jun;152(Pt 6):1575-1580 PMID: 16735721
  28. Glycoproteomics based on tandem mass spectrometry of glycopeptides.
    J Chromatogr B Analyt Technol Biomed Life Sci. 2007 Apr 15;849(1-2):115-28 PMID: 17049937
  29. Glycomic profiling of cells and tissues by mass spectrometry: fingerprinting and sequencing methodologies.
    Methods Enzymol. 2006;415:59-86 PMID: 17116468
  30. FAB-mapping of recombinant-DNA protein products.
    Biochem Biophys Res Commun. 1983 Nov 30;117(1):299-305 PMID: 6661227
  31. The cytoplasmic F-box binding protein SKP1 contains a novel pentasaccharide linked to hydroxyproline in Dictyostelium.
    J Biol Chem. 1998 Jul 17;273(29):18242-9 PMID: 9660787
  32. Structural analysis of the oligosaccharides derived from glycodelin, a human glycoprotein with potent immunosuppressive and contraceptive activities.
    J Biol Chem. 1995 Oct 13;270(41):24116-26 PMID: 7592613
  33. Rapid identification of proteins by peptide-mass fingerprinting.
    Curr Biol. 1993 Jun 1;3(6):327-32 PMID: 15335725
  34. Structural characterization of the N-glycans of gp273, the ligand for sperm-egg interaction in the mollusc bivalve Unio elongatulus.
    Glycoconj J. 2001 Jul;18(7):511-8 PMID: 12151712
  35. Gp273, the ligand molecule for sperm-egg interaction in the bivalve mollusk, Unio elongatulus, binds to and induces acrosome reaction in human spermatozoa through a protein kinase C-dependent pathway.
    Biol Reprod. 2003 Dec;69(6):1779-84 PMID: 12890719
  36. N-glycoproteomics - an automated workflow approach.
    Glycobiology. 2008 Apr;18(4):339-49 PMID: 18272656
  37. Antifreeze glycoproteins from the blood of an antarctic fish. The structure of the proline-containing glycopeptides.
    J Biol Chem. 1978 Jul 25;253(14):5155-62 PMID: 670183
  38. Strategies for analysis of glycoprotein glycosylation.
    Biochim Biophys Acta. 2006 Dec;1764(12):1853-69 PMID: 17134948
  39. Immunoglobulins against gp273, the ligand for sperm-egg interaction in the mollusc bivalve Unio elongatulus, are directed against charged O-linked oligosaccharide chains bearing a Lewis-like structure and interact with epitopes of the human zona pellucida.
    Mol Reprod Dev. 2003 Feb;64(2):226-34 PMID: 12506356
  40. Glycomics and proteomics analyses of mouse uterine luminal fluid revealed a predominance of Lewis Y and X epitopes on specific protein carriers.
    Mol Cell Proteomics. 2009 Feb;8(2):325-42 PMID: 18941134
  41. Gender-specific glycosylation of human glycodelin affects its contraceptive activity.
    J Biol Chem. 1996 Dec 13;271(50):32159-67 PMID: 8943270
  42. A tetraantennary glycan with bisecting N-acetylglucosamine and the Sd(a) antigen is the predominant N-glycan on bovine pregnancy-associated glycoproteins.
    Glycobiology. 2008 Jan;18(1):42-52 PMID: 17951374
  43. Lectins: past, present and future.
    Biochem Soc Trans. 2008 Dec;36(Pt 6):1457-60 PMID: 19021575
Article Info
Journal
FEBS letters
Abbr.
FEBS Lett
ISSN
1873-3468
DOI
10.1016/j.febslet.2009.03.049
Published
2009-06-05
Epub
2009-00-27
Pages
1728-35
Language
English
Region
England
NLM ID
0155157
PMCID
PMC2753369
Subset
IM
Grants
NIGMS NIH HHS · U54 GM062116-01A1 · United States
NIGMS NIH HHS · U54 GM062116-069002 · United States
Biotechnology and Biological Sciences Research Council · B19088 · United Kingdom
Biotechnology and Biological Sciences Research Council · BBF0083091 · United Kingdom
NIGMS NIH HHS · U54 GM062116 · United States
NIGMS NIH HHS · U54 GM062116-089002 · United States
NIGMS NIH HHS · U54 GM062116-079002 · United States
External Links
PubMed source DOI: 10.1016/j.febslet.2009.03.049 PMC Full Text View journal details
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