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PMID: 19337404 Published · ppublish English Journal Article

VEGF and endothelial guidance in angiogenic sprouting.

Organogenesis ·Vol. 4 ·No. 4 ·2008-10-00 ·Pages 241-6

Gerhardt H

Abstract

The cellular actions of VEGF need to be coordinated to guide vascular patterning during sprouting angiogenesis. Individual endothelial tip cells lead and guide the blood vessel sprout, while neighbouring stalk cells proliferate and form the vascular lumen. Recent studies illustrate how endothelial DLL4/NOTCH signalling, stimulated by VEGF, regulates the sprouting response by limiting tip cell formation in the stalk. The spatial distribution of VEGF, in turn, regulates the shape of the ensuing sprout by directing tip cell migration and determining stalk cell proliferation.

Keywords
Notch VEGF angiogenesis tip cells vascular patterning
Authors & Affiliations
1 authors, click to expand affiliations / ORCID
Gerhardt Holger
Vascular Biology Laboratory; London Research Institute; Cancer Research UK; Lincoln's Inn Fields Laboratories; London UK.
References (48)
48 references, click to expand
  1. Angiogenesis in the mouse retina: a model system for experimental manipulation.
    Exp Cell Res. 2006 Mar 10;312(5):676-83 PMID: 16337189
  2. Development of the human retinal vasculature: cellular relations and VEGF expression.
    Exp Eye Res. 1997 Oct;65(4):555-68 PMID: 9464188
  3. Blockade of Dll4 inhibits tumour growth by promoting non-productive angiogenesis.
    Nature. 2006 Dec 21;444(7122):1032-7 PMID: 17183313
  4. Mechanisms of angiogenesis.
    Nature. 1997 Apr 17;386(6626):671-4 PMID: 9109485
  5. Stabilization of the retinal vascular network by reciprocal feedback between blood vessels and astrocytes.
    Development. 2005 Apr;132(8):1855-62 PMID: 15790963
  6. Dll4 signalling through Notch1 regulates formation of tip cells during angiogenesis.
    Nature. 2007 Feb 15;445(7129):776-80 PMID: 17259973
  7. First blood vessels in the avian neural tube are formed by a combination of dorsal angioblast immigration and ventral sprouting of endothelial cells.
    Dev Biol. 1996 Jan 10;173(1):133-47 PMID: 8575615
  8. VEGF guides angiogenic sprouting utilizing endothelial tip cell filopodia.
    J Cell Biol. 2003 Jun 23;161(6):1163-77 PMID: 12810700
  9. Delta-like ligand 4 (Dll4) is induced by VEGF as a negative regulator of angiogenic sprouting.
    Proc Natl Acad Sci U S A. 2007 Feb 27;104(9):3219-24 PMID: 17296940
  10. Shear stress regulates forward and reverse planar cell polarity of vascular endothelium in vivo and in vitro.
    Circ Res. 2006 Apr 14;98(7):939-46 PMID: 16527990
  11. Vascular endothelial growth factor receptor Flt-1 negatively regulates developmental blood vessel formation by modulating endothelial cell division.
    Blood. 2002 Apr 1;99(7):2397-407 PMID: 11895772
  12. Genetic control of branching morphogenesis.
    Science. 1999 Jun 4;284(5420):1635-9 PMID: 10383344
  13. Cell-autonomous notch signaling regulates endothelial cell branching and proliferation during vascular tubulogenesis.
    FASEB J. 2005 Jun;19(8):1027-9 PMID: 15774577
  14. In vivo imaging reveals different cellular functions for FGF and Dpp signaling in tracheal branching morphogenesis.
    Dev Cell. 2002 May;2(5):677-83 PMID: 12015974
  15. The formation of capillary basement membranes during internal vascularization of the rat's cerebral cortex.
    Z Zellforsch Mikrosk Anat. 1972;133(2):231-48 PMID: 5082884
  16. Dosage-sensitive requirement for mouse Dll4 in artery development.
    Genes Dev. 2004 Oct 15;18(20):2474-8 PMID: 15466159
  17. Notch signaling pathway.
    Sci STKE. 2006 Dec 05;2006(364):cm7 PMID: 17148788
  18. HES and HERP families: multiple effectors of the Notch signaling pathway.
    J Cell Physiol. 2003 Mar;194(3):237-55 PMID: 12548545
  19. Spatially restricted patterning cues provided by heparin-binding VEGF-A control blood vessel branching morphogenesis.
    Genes Dev. 2002 Oct 15;16(20):2684-98 PMID: 12381667
  20. Periodic Delta-like 4 expression in developing retinal arteries.
    Gene Expr Patterns. 2004 Nov;5(1):123-7 PMID: 15533827
  21. A presenilin-1-dependent gamma-secretase-like protease mediates release of Notch intracellular domain.
    Nature. 1999 Apr 8;398(6727):518-22 PMID: 10206645
  22. Notch signalling limits angiogenic cell behaviour in developing zebrafish arteries.
    Nature. 2007 Feb 15;445(7129):781-4 PMID: 17259972
  23. Migration and proliferation of endothelial cells in preformed and newly formed blood vessels during tumor angiogenesis.
    Microvasc Res. 1977 Jul;14(1):53-65 PMID: 895546
  24. The Notch ligand Delta-like 4 negatively regulates endothelial tip cell formation and vessel branching.
    Proc Natl Acad Sci U S A. 2007 Feb 27;104(9):3225-30 PMID: 17296941
  25. Regulation of Notch1 and Dll4 by vascular endothelial growth factor in arterial endothelial cells: implications for modulating arteriogenesis and angiogenesis.
    Mol Cell Biol. 2003 Jan;23(1):14-25 PMID: 12482957
  26. Endothelial cell-astrocyte interactions. A cellular model of the blood-brain barrier.
    Ann N Y Acad Sci. 1988;529:31-9 PMID: 3293508
  27. Retinal vascular development is mediated by endothelial filopodia, a preexisting astrocytic template and specific R-cadherin adhesion.
    Invest Ophthalmol Vis Sci. 2002 Nov;43(11):3500-10 PMID: 12407162
  28. Astrocyte-endothelial interactions at the blood-brain barrier.
    Nat Rev Neurosci. 2006 Jan;7(1):41-53 PMID: 16371949
  29. Ultrastructural observation on the tips of growing vascular cords in the rat cerebral cortex.
    Experientia. 1982 Apr 15;38(4):499-501 PMID: 7084422
  30. Rapid Notch1 nuclear translocation after ligand binding depends on presenilin-associated gamma-secretase activity.
    Ann N Y Acad Sci. 2000;920:223-6 PMID: 11193154
  31. Oxygen modifies artery differentiation and network morphogenesis in the retinal vasculature.
    Dev Dyn. 2005 Jul;233(3):822-8 PMID: 15895398
  32. Development of retinal vasculature is mediated by hypoxia-induced vascular endothelial growth factor (VEGF) expression by neuroglia.
    J Neurosci. 1995 Jul;15(7 Pt 1):4738-47 PMID: 7623107
  33. Development of the retinal vasculature.
    Angiogenesis. 2007;10(2):77-88 PMID: 17322966
  34. A new model of vasculogenesis and angiogenesis in vitro as compared with vascular growth in the avian area vasculosa.
    Anat Rec. 1993 Sep;237(1):49-57 PMID: 7692767
  35. Vascular endothelial growth factor is a secreted angiogenic mitogen.
    Science. 1989 Dec 8;246(4935):1306-9 PMID: 2479986
  36. Social interactions among epithelial cells during tracheal branching morphogenesis.
    Nature. 2006 Jun 8;441(7094):746-9 PMID: 16760977
  37. Orientation of endothelial cell division is regulated by VEGF signaling during blood vessel formation.
    Blood. 2007 Feb 15;109(4):1345-52 PMID: 17068148
  38. Inhibition of Dll4 signalling inhibits tumour growth by deregulating angiogenesis.
    Nature. 2006 Dec 21;444(7122):1083-7 PMID: 17183323
  39. PDGF mediates a neuron-astrocyte interaction in the developing retina.
    Neuron. 1996 Dec;17(6):1117-31 PMID: 8982160
  40. Studies on the vasculogenesis in rat cerebral cortex.
    Anat Rec. 1989 Jul;224(3):355-64 PMID: 2782620
  41. Endothelial signalling by the Notch ligand Delta-like 4 restricts angiogenesis.
    Development. 2007 Mar;134(5):839-44 PMID: 17251261
  42. Growing and shaping the vascular tree: multiple roles for VEGF.
    Bioessays. 2003 Nov;25(11):1052-60 PMID: 14579246
  43. Dll4, a novel Notch ligand expressed in arterial endothelium.
    Genes Dev. 2000 Jun 1;14(11):1313-8 PMID: 10837024
  44. The VEGF receptor flt-1 (VEGFR-1) is a positive modulator of vascular sprout formation and branching morphogenesis.
    Blood. 2004 Jun 15;103(12):4527-35 PMID: 14982871
  45. Haploinsufficient lethality and formation of arteriovenous malformations in Notch pathway mutants.
    Genes Dev. 2004 Oct 15;18(20):2469-73 PMID: 15466160
  46. Early vascularization of the embryonic cerebral cortex: Golgi and electron microscopic studies.
    J Comp Neurol. 1985 Nov 8;241(2):237-49 PMID: 4067017
  47. Haploinsufficiency of delta-like 4 ligand results in embryonic lethality due to major defects in arterial and vascular development.
    Proc Natl Acad Sci U S A. 2004 Nov 9;101(45):15949-54 PMID: 15520367
  48. The role of vascular endothelial growth factor in blood vessel formation.
    Trends Cell Biol. 1996 Dec;6(12):454-6 PMID: 15157501
Article Info
Journal
Organogenesis
Abbr.
Organogenesis
ISSN
1547-6278
DOI
10.4161/org.4.4.7414
Published
2008-10-00
Pages
241-6
Language
English
Region
United States
NLM ID
101253266
PMCID
PMC2634329
External Links
PubMed source DOI: 10.4161/org.4.4.7414 PMC Full Text View journal details
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