Home LiteratureArticle Details
PMID: 19346354 Published · ppublish English Comparative Study Journal Article

Genome-scale model of Streptococcus thermophilus LMG18311 for metabolic comparison of lactic acid bacteria.

Applied and environmental microbiology ·Vol. 75 ·No. 11 ·2009-06-00 ·Pages 3627-33

Pastink MI, Teusink B, Hols P, Visser S, de Vos WM, Hugenholtz J

Abstract

In this report, we describe the amino acid metabolism and amino acid dependency of the dairy bacterium Streptococcus thermophilus LMG18311 and compare them with those of two other characterized lactic acid bacteria, Lactococcus lactis and Lactobacillus plantarum. Through the construction of a genome-scale metabolic model of S. thermophilus, the metabolic differences between the three bacteria were visualized by direct projection on a metabolic map. The comparative analysis revealed the minimal amino acid auxotrophy (only histidine and methionine or cysteine) of S. thermophilus LMG18311 and the broad variety of volatiles produced from amino acids compared to the other two bacteria. It also revealed the limited number of pyruvate branches, forcing this strain to use the homofermentative metabolism for growth optimization. In addition, some industrially relevant features could be identified in S. thermophilus, such as the unique pathway for acetaldehyde (yogurt flavor) production and the absence of a complete pentose phosphate pathway.

MeSH Terms
Acetaldehyde/metabolism Amino Acids/metabolism Lactobacillus plantarum/metabolism Lactococcus lactis/metabolism Metabolic Networks and Pathways/genetics Metabolome Models, Biological Pyruvates/metabolism Streptococcus thermophilus/genetics,metabolism
Chemicals
Amino Acids Pyruvates Acetaldehyde
Authors & Affiliations
6 authors, click to expand affiliations / ORCID
Pastink Margreet I
Top Institute Food and Nutrition, Nieuwe Kanaal, PA Wageningen, The Netherlands.
Teusink Bas
Hols Pascal
Visser Sanne
de Vos Willem M
Hugenholtz Jeroen
References (29)
29 references, click to expand
  1. Diversity of L-leucine catabolism in various microorganisms involved in dairy fermentations, and identification of the rate-controlling step in the formation of the potent flavour component 3-methylbutanal.
    Appl Microbiol Biotechnol. 2004 Apr;64(3):396-402 PMID: 14624315
  2. Natural diversity and adaptive responses of Lactococcus lactis.
    Curr Opin Biotechnol. 2006 Apr;17(2):183-90 PMID: 16517150
  3. Untargeted large-scale plant metabolomics using liquid chromatography coupled to mass spectrometry.
    Nat Protoc. 2007;2(4):778-91 PMID: 17446877
  4. Reconstructing the metabolic network of a bacterium from its genome.
    Trends Microbiol. 2005 Nov;13(11):550-8 PMID: 16169729
  5. An untargeted metabolomics approach to contaminant analysis: pinpointing potential unknown compounds.
    Anal Chim Acta. 2007 Feb 12;584(1):43-9 PMID: 17386583
  6. Complete genome sequence of the prototype lactic acid bacterium Lactococcus lactis subsp. cremoris MG1363.
    J Bacteriol. 2007 Apr;189(8):3256-70 PMID: 17307855
  7. Diversity analysis of dairy and nondairy Lactococcus lactis isolates, using a novel multilocus sequence analysis scheme and (GTG)5-PCR fingerprinting.
    Appl Environ Microbiol. 2007 Nov;73(22):7128-37 PMID: 17890345
  8. Citrate Fermentation by Lactococcus and Leuconostoc spp.
    Appl Environ Microbiol. 1991 Dec;57(12):3535-40 PMID: 16348602
  9. Complete sequence and comparative genome analysis of the dairy bacterium Streptococcus thermophilus.
    Nat Biotechnol. 2004 Dec;22(12):1554-8 PMID: 15543133
  10. New insights in the molecular biology and physiology of Streptococcus thermophilus revealed by comparative genomics.
    FEMS Microbiol Rev. 2005 Aug;29(3):435-63 PMID: 16125007
  11. Metabolic engineering of acetaldehyde production by Streptococcus thermophilus.
    Appl Environ Microbiol. 2002 Nov;68(11):5656-62 PMID: 12406762
  12. An aminotransferase from Lactococcus lactis initiates conversion of amino acids to cheese flavor compounds.
    Appl Environ Microbiol. 1997 Feb;63(2):414-9 PMID: 9023921
  13. Genome-scale models of microbial cells: evaluating the consequences of constraints.
    Nat Rev Microbiol. 2004 Nov;2(11):886-97 PMID: 15494745
  14. Complete genome sequence of Lactobacillus plantarum WCFS1.
    Proc Natl Acad Sci U S A. 2003 Feb 18;100(4):1990-5 PMID: 12566566
  15. High-level acetaldehyde production in Lactococcus lactis by metabolic engineering.
    Appl Environ Microbiol. 2005 Feb;71(2):1109-13 PMID: 15691976
  16. Branched-chain amino acid biosynthesis is essential for optimal growth of Streptococcus thermophilus in milk.
    Appl Environ Microbiol. 2000 Dec;66(12):5128-33 PMID: 11097879
  17. Molecular characterization, over-expression and purification of a novel dipeptidase from Lactobacillus helveticus.
    Appl Microbiol Biotechnol. 1996 Jun;45(5):638-45 PMID: 8766699
  18. Metabolism and Energetics of Lactococcus lactis during Growth in Complex or Synthetic Media.
    Appl Environ Microbiol. 1997 Jul;63(7):2665-70 PMID: 16535643
  19. Modeling Lactococcus lactis using a genome-scale flux model.
    BMC Microbiol. 2005 Jun 27;5:39 PMID: 15982422
  20. Development of a minimal chemically-defined medium for the exponential growth of Streptococcus thermophilus.
    J Appl Microbiol. 2001 Dec;91(6):1023-9 PMID: 11851809
  21. Amino acid requirements and peptidase activities of Streptococcus salivarius subsp. thermophilus.
    J Appl Bacteriol. 1995 Sep;79(3):302-7 PMID: 7592124
  22. Accelerating the reconstruction of genome-scale metabolic networks.
    BMC Bioinformatics. 2006 Jun 13;7:296 PMID: 16772023
  23. Minimal Requirements for Exponential Growth of Lactococcus lactis.
    Appl Environ Microbiol. 1993 Dec;59(12):4363-6 PMID: 16349136
  24. Cooperation between Lactococcus lactis and nonstarter lactobacilli in the formation of cheese aroma from amino acids.
    Appl Environ Microbiol. 2003 Feb;69(2):734-9 PMID: 12570989
  25. Analysis of growth of Lactobacillus plantarum WCFS1 on a complex medium using a genome-scale metabolic model.
    J Biol Chem. 2006 Dec 29;281(52):40041-8 PMID: 17062565
  26. Control of the shift from homolactic acid to mixed-acid fermentation in Lactococcus lactis: predominant role of the NADH/NAD+ ratio.
    J Bacteriol. 1997 Sep;179(17):5282-7 PMID: 9286977
  27. In silico reconstruction of the metabolic pathways of Lactobacillus plantarum: comparing predictions of nutrient requirements with those from growth experiments.
    Appl Environ Microbiol. 2005 Nov;71(11):7253-62 PMID: 16269766
  28. The complete genome sequence of Lactobacillus bulgaricus reveals extensive and ongoing reductive evolution.
    Proc Natl Acad Sci U S A. 2006 Jun 13;103(24):9274-9 PMID: 16754859
  29. Relation of growth of Streptococcus lactis and Streptococcus cremoris to amino acid transport.
    J Bacteriol. 1988 Feb;170(2):700-7 PMID: 3123462
Article Info
Journal
Applied and environmental microbiology
Abbr.
Appl Environ Microbiol
ISSN
1098-5336
DOI
10.1128/AEM.00138-09
Published
2009-06-00
Epub
2009-00-03
Pages
3627-33
Language
English
Region
United States
NLM ID
7605801
PMCID
PMC2687286
Subset
IM
External Links
PubMed source DOI: 10.1128/AEM.00138-09 PMC Full Text View journal details
Analysis Services
Analysis Services

Contact

No. 2 Wenbo Road, Zhangqiu District, Jinan, Shandong

Qilu Normal University · Genelibs Bioinformatics Lab

750 Shunhua Rd, Jinan

2F, Bldg F, University Science Park

Tel: 0531-88819269

WeChat Official Account

Follow our WeChat subscription account for real-time updates and the latest in medical and biological research.


Business Email

E-mail: [email protected]

Copyright © 2026.Genelibs Jinan Honghui Biotechnology Co., Ltd. 鲁ICP备20007159号-2