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PMID: 1935893 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Physical map of the 3' region of the human immunoglobulin heavy chain locus: clustering of autoantibody-related variable segments in one haplotype.

The EMBO journal ·Vol. 10 ·No. 12 ·1991-12-00 ·Pages 3641-5

Shin EK, Matsuda F, Nagaoka H, Fukita Y, Imai T, Yokoyama K, Soeda E, Honjo T

Abstract

We have constructed the physical map of the 3' region of the human immunoglobulin heavy chain variable region (VH) genes. DNA segments extending to 200 kb upstream of the JH segment were isolated in two YAC clones. Five VH segments were identified in this region in the 5' to 3' order, V(II-5), V(IV-4), V(I-3), V(I-2), and V(VI-1) segments which were all structurally normal and orientated in the same direction as the JH segments. From DNA of a different cell line we have isolated a cosmid contig containing the same DNA region which has extraordinary polymorphism. The YAC and cosmid DNAs were called haplotypes A and B, respectively. Haplotype B contained an additional VH-I segment (V(I-4.1b)) between the V(II-5) and V(IV-4) segments. V(I-4.1b) segment is almost identical to a previously published VH sequence encoding a rheumatoid factor. Another VH segment in the B haplotype (V(I-3b)) corresponding to the V(I-3) segment also showed 99.7% nucleotide sequence homology with an anti-DNA autoantibody VH sequence. However, none of the VH sequences in haplotype A showed such strong homology with autoantibody VH sequences. The results suggest that VH haplotypes may have linkage with autoantibody production.

MeSH Terms
Amino Acid Sequence Autoantibodies/genetics Base Sequence Blotting, Southern Chromosomes, Fungal Cosmids DNA/genetics Gene Library Genome, Human Haplotypes Humans Immunoglobulin Heavy Chains/genetics Immunoglobulin Variable Region/genetics Molecular Sequence Data Pedigree Polymorphism, Genetic Restriction Mapping
Chemicals
Autoantibodies Immunoglobulin Heavy Chains Immunoglobulin Variable Region DNA
Authors & Affiliations
8 authors, click to expand affiliations / ORCID
Shin E K
Department of Medical Chemistry, Faculty of Medicine, Kyoto University, Japan.
Matsuda F
Nagaoka H
Fukita Y
Imai T
Yokoyama K
Soeda E
Honjo T
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Article Info
Journal
The EMBO journal
Abbr.
EMBO J
ISSN
0261-4189
Published
1991-12-00
Pages
3641-5
Language
English
Region
England
NLM ID
8208664
PMCID
PMC453095
Subset
IM
Databases
GENBANK
X62106, X62107, X62108, X62109, X62110, X62111, X62112
External Links
PubMed source PMC Full Text View journal details
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