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PMID: 19363482 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Insights into substrate stabilization from snapshots of the peptidyl transferase center of the intact 70S ribosome.

Nature structural & molecular biology ·Vol. 16 ·No. 5 ·2009-05-00 ·Pages 528-33

Voorhees RM, Weixlbaumer A, Loakes D, Kelley AC, Ramakrishnan V

Abstract

Protein synthesis is catalyzed in the peptidyl transferase center (PTC), located in the large (50S) subunit of the ribosome. No high-resolution structure of the intact ribosome has contained a complete active site including both A- and P-site tRNAs. In addition, although past structures of the 50S subunit have found no ordered proteins at the PTC, biochemical evidence suggests that specific proteins are capable of interacting with the 3' ends of tRNA ligands. Here we present structures, at 3.6-A and 3.5-A resolution respectively, of the 70S ribosome in complex with A- and P-site tRNAs that mimic pre- and post-peptidyl-transfer states. These structures demonstrate that the PTC is very similar between the 50S subunit and the intact ribosome. They also reveal interactions between the ribosomal proteins L16 and L27 and the tRNA substrates, helping to elucidate the role of these proteins in peptidyl transfer.

MeSH Terms
Crystallography, X-Ray Escherichia coli Peptidyl Transferases/metabolism Protein Binding Protein Structure, Secondary RNA, Ribosomal, 23S/chemistry,metabolism RNA, Transfer/chemistry,metabolism Ribosomal Proteins/chemistry,metabolism Ribosomes/enzymology Static Electricity Substrate Specificity Thermus thermophilus/metabolism
Chemicals
RNA, Ribosomal, 23S Ribosomal Proteins ribosomal protein L16 ribosomal proteins L27 RNA, Transfer Peptidyl Transferases
Authors & Affiliations
5 authors, click to expand affiliations / ORCID
Voorhees Rebecca M
Medical Research Council Laboratory of Molecular Biology, Cambridge, UK.
Weixlbaumer Albert
Loakes David
Kelley Ann C
Ramakrishnan V
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Article Info
Journal
Nature structural & molecular biology
Abbr.
Nat Struct Mol Biol
ISSN
1545-9985
DOI
10.1038/nsmb.1577
Published
2009-05-00
Epub
2009-00-12
Pages
528-33
Language
English
Region
United States
NLM ID
101186374
PMCID
PMC2679717
Subset
IM
Grants
Wellcome Trust · 082086 · United Kingdom
Medical Research Council · MC_U105184332 · United Kingdom
Medical Research Council · U.1051.04.018(78935) · United Kingdom
Databases
PDB
2WDG, 2WDH, 2WDI, 2WDJ, 2WDK, 2WDL, 2WDM, 2WDN
External Links
PubMed source DOI: 10.1038/nsmb.1577 PMC Full Text View journal details
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