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PMID: 19377485 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Universal sample preparation method for proteome analysis.

Nature methods ·Vol. 6 ·No. 5 ·2009-05-00 ·Pages 359-62

Wiśniewski JR, Zougman A, Nagaraj N, Mann M

Abstract

We describe a method, filter-aided sample preparation (FASP), which combines the advantages of in-gel and in-solution digestion for mass spectrometry-based proteomics. We completely solubilized the proteome in sodium dodecyl sulfate, which we then exchanged by urea on a standard filtration device. Peptides eluted after digestion on the filter were pure, allowing single-run analyses of organelles and an unprecedented depth of proteome coverage.

MeSH Terms
Analytic Sample Preparation Methods/methods Animals Brain Chemistry Endopeptidases/chemistry HeLa Cells Humans Iodoacetamide/chemistry Liver/chemistry Mass Spectrometry/methods Mice Mitochondria/chemistry Proteome/analysis,chemistry Proteomics/methods Sodium Dodecyl Sulfate/chemistry Tandem Mass Spectrometry/methods Ultrafiltration/methods Urea/chemistry
Chemicals
Proteome Sodium Dodecyl Sulfate Urea Endopeptidases Iodoacetamide
Authors & Affiliations
4 authors, click to expand affiliations / ORCID
Wiśniewski Jacek R
Department of Proteomics and Signal Transduction, Max Planck Institute for Biochemistry, Martinsried, Germany. [email protected]
Zougman Alexandre
Nagaraj Nagarjuna
Mann Matthias
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Article Info
Journal
Nature methods
Abbr.
Nat Methods
ISSN
1548-7105
DOI
10.1038/nmeth.1322
Email
[email protected]
Published
2009-05-00
Epub
2009-00-19
Pages
359-62
Language
English
Region
United States
NLM ID
101215604
Subset
IM
Corrections
CommentIn
PMID: 19876013
External Links
PubMed source DOI: 10.1038/nmeth.1322 View journal details
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