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PMID: 1937772 Published · ppublish English Journal Article

A new in vitro model of Entamoeba histolytica adhesion, using the human colon carcinoma cell line Caco-2: scanning electron microscopic study.

Infection and immunity ·Vol. 59 ·No. 11 ·1991-11-00 ·Pages 4142-6

Rigothier MC, Coconnier MH, Servin AL, Gayral P

Abstract

The human colon carcinoma cell line Caco-2, which is widely used to study the adhesion and cytotoxicity of enterobacteria, was used to investigate the adhesion of the trophozoites of Entamoeba histolytica. We observed a high percentage of adhesion of amoebae to Caco-2 cells. Scanning electron microscopy showed that amoebial membrane structures were involved in adhesion and the cytolytic action. These differentiated cells should prove to be a useful model system for investigation of the pathogenic action of amoebae.

MeSH Terms
Animals Cell Adhesion Colonic Neoplasms/pathology Dysentery, Amebic/pathology Entamoeba histolytica/pathogenicity Humans In Vitro Techniques Intestinal Mucosa/parasitology Microscopy, Electron, Scanning Tumor Cells, Cultured
Authors & Affiliations
4 authors, click to expand affiliations / ORCID
Rigothier M C
Laboratoire de Biologie et Contrôle des Organismes Parasites, Université de Paris XI, Châtenay-Malabry, France.
Coconnier M H
Servin A L
Gayral P
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Article Info
Journal
Infection and immunity
Abbr.
Infect Immun
ISSN
0019-9567
DOI
10.1128/iai.59.11.4142-4146.1991
Published
1991-11-00
Pages
4142-6
Language
English
Region
United States
NLM ID
0246127
PMCID
PMC259008
Subset
IM
External Links
PubMed source DOI: 10.1128/iai.59.11.4142-4146.1991 PMC Full Text View journal details
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