Sexual stage development of cryptosporidia in the Caco-2 cell line.

Infection and immunity ·Vol. 59 ·No. 12 ·1991-12-00 ·Pages 4610-3

Buraud M, Forget E, Favennec L, Bizet J, Gobert JG, Deluol AM

Abstract

We used the spontaneously differentiated human intestinal epithelial cell line Caco-2 to develop an in vitro model of Cryptosporidium sp. infection. The mean cell infection rate was 3% +/- 2%. Asexual stages of cryptosporidia were observed on day 2 postinoculation. Transmission electron microscopy showed the presence of macrogametes at day 5. This cell line appears to be suited to the study of the mechanisms by which biological agents inhibit both sexual and asexual development of cryptosporidia.

MeSH Terms

Animals Cell Line Cryptosporidium/growth & development,ultrastructure Intestines/parasitology

Authors & Affiliations

6 authors, click to expand affiliations / ORCID

Buraud M

Laboratoire de Biologie Animale et Parasitaire, Faculté des Sciences Pharmaceutiques et Biologiques, 4, Paris, France.

Forget E

Favennec L

Bizet J

Gobert J G

Deluol A M

References (6)

6 references, click to expand

Development of human and calf Cryptosporidium in chicken embryos.
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Complete development of Cryptosporidium in cell culture.
Science. 1984 May 11;224(4649):603-5 PMID: 6710159
Asexual development of Cryptosporidium parvum within a differentiated human enterocyte cell line.
Infect Immun. 1991 Jan;59(1):234-9 PMID: 1987037
Cryptosporidiosis in perspective.
Adv Parasitol. 1988;27:63-129 PMID: 3289331
In vitro cultivation of Cryptosporidium parvum and screening for anticryptosporidial drugs.
Antimicrob Agents Chemother. 1990 Aug;34(8):1498-500 PMID: 2221857
Isolation of Cryptosporidium oocysts and sporozoites using discontinuous sucrose and isopycnic Percoll gradients.
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Article Info

Journal

Infection and immunity

Abbr.

Infect Immun

ISSN

0019-9567

DOI

10.1128/iai.59.12.4610-4613.1991

Published

1991-12-00

Pages

4610-3

Language

English

Region

United States

NLM ID

0246127

PMCID

PMC259085

Subset

External Links

PubMed source DOI: 10.1128/iai.59.12.4610-4613.1991 PMC Full Text View journal details

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Sexual stage development of cryptosporidia in the Caco-2 cell line.

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