Home LiteratureArticle Details
PMID: 1938905 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, Non-P.H.S. Research Support, U.S. Gov't, P.H.S.

Fragments of the internal transcribed spacer 1 of pre-rRNA accumulate in Saccharomyces cerevisiae lacking 5'----3' exoribonuclease 1.

Journal of bacteriology ·Vol. 173 ·No. 21 ·1991-11-00 ·Pages 7024-8

Stevens A, Hsu CL, Isham KR, Larimer FW

Abstract

The portion of the internal transcribed spacer 1 found on 20S pre-rRNA accumulates in Saccharomyces cerevisiae lacking 5'----3' exoribonuclease 1, showing that an endonucleolytic cleavage at the 3' terminus of 18S rRNA is involved in the 20S pre-rRNA to 18S mature rRNA conversion. Smaller fragments of the spacer sequence are also found. The exoribonuclease may be involved as a cytoplasmic RNase in the hydrolysis of the spacer.

MeSH Terms
Exoribonucleases/metabolism RNA Precursors/metabolism RNA Processing, Post-Transcriptional RNA, Ribosomal, 18S/metabolism Saccharomyces cerevisiae/enzymology,genetics
Chemicals
RNA Precursors RNA, Ribosomal, 18S Exoribonucleases exoribonuclease II
Authors & Affiliations
4 authors, click to expand affiliations / ORCID
Stevens A
Biology Division, Oak Ridge National Laboratory, Tennessee 37831-8077.
Hsu C L
Isham K R
Larimer F W
References (19)
19 references, click to expand
  1. Isolation and characterization of yeast ribosomal RNA precursors and preribosomes.
    Methods Enzymol. 1989;180:96-109 PMID: 2693913
  2. Accurate processing of human pre-rRNA in vitro.
    Mol Cell Biol. 1989 Oct;9(10):4422-31 PMID: 2586517
  3. Disruption of the gene XRN1, coding for a 5'----3' exoribonuclease, restricts yeast cell growth.
    Gene. 1990 Oct 30;95(1):85-90 PMID: 1979303
  4. kem mutations affect nuclear fusion in Saccharomyces cerevisiae.
    Genetics. 1990 Dec;126(4):799-812 PMID: 2076815
  5. Labeling of RNA and phosphoproteins in Saccharomyces cerevisiae.
    Methods Enzymol. 1991;194:423-8 PMID: 2005801
  6. Cloning and characterization of DST2, the gene for DNA strand transfer protein beta from Saccharomyces cerevisiae.
    Mol Cell Biol. 1991 May;11(5):2583-92 PMID: 1850100
  7. Molecular and genetic analysis of the gene encoding the Saccharomyces cerevisiae strand exchange protein Sep1.
    Mol Cell Biol. 1991 May;11(5):2593-608 PMID: 1840632
  8. The cytoplasmic maturation of a ribosomal precursor ribonucleic acid in yeast.
    J Biol Chem. 1973 Feb 25;248(4):1412-6 PMID: 4568815
  9. Terminal nucleotide sequences of 17-S ribosomal RNA and its immediate precursor 18-S RNA in yeast.
    Eur J Biochem. 1977 Jan;72(2):361-9 PMID: 190001
  10. Purification and characterization of a Saccharomyces cerevisiae exoribonuclease which yields 5'-mononucleotides by a 5' leads to 3' mode of hydrolysis.
    J Biol Chem. 1980 Apr 10;255(7):3080-5 PMID: 6244307
  11. Some characteristics of processing sites in ribosomal precursor RNA of yeast.
    Nucleic Acids Res. 1980 Jul 11;8(13):2907-20 PMID: 6253896
  12. Location of the initial cleavage sites in mouse pre-rRNA.
    Mol Cell Biol. 1983 Aug;3(8):1501-10 PMID: 6621536
  13. Genomic sequencing.
    Proc Natl Acad Sci U S A. 1984 Apr;81(7):1991-5 PMID: 6326095
  14. Isolation and properties of a single-strand 5'----3' exoribonuclease from Ehrlich ascites tumor cell nucleoli.
    Biochemistry. 1984 Sep 11;23(19):4367-73 PMID: 6207856
  15. Lariat structures are in vivo intermediates in yeast pre-mRNA splicing.
    Cell. 1984 Dec;39(3 Pt 2):611-21 PMID: 6096014
  16. A 5'----3' exoribonuclease of Saccharomyces cerevisiae: size and novel substrate specificity.
    Arch Biochem Biophys. 1987 Feb 1;252(2):339-47 PMID: 3545079
  17. Purification and characterization of an activity from Saccharomyces cerevisiae that catalyzes homologous pairing and strand exchange.
    Proc Natl Acad Sci U S A. 1987 Aug;84(16):5560-4 PMID: 3303029
  18. Transcription and processing of RNA from mouse ribosomal DNA transfected into hamster cells.
    Mol Cell Biol. 1989 Apr;9(4):1667-71 PMID: 2725522
  19. DNA strand transfer protein beta from yeast mitotic cells differs from strand transfer protein alpha from meiotic cells.
    J Biol Chem. 1990 Jul 5;265(19):10968-73 PMID: 2141605
Article Info
Journal
Journal of bacteriology
Abbr.
J Bacteriol
ISSN
0021-9193
DOI
10.1128/jb.173.21.7024-7028.1991
Published
1991-11-00
Pages
7024-8
Language
English
Region
United States
NLM ID
2985120R
PMCID
PMC209060
Subset
IM
Grants
NCI NIH HHS · CA 09336 · United States
NIGMS NIH HHS · GM42144 · United States
External Links
PubMed source DOI: 10.1128/jb.173.21.7024-7028.1991 PMC Full Text View journal details
Analysis Services
Analysis Services

Contact

No. 2 Wenbo Road, Zhangqiu District, Jinan, Shandong

Qilu Normal University · Genelibs Bioinformatics Lab

750 Shunhua Rd, Jinan

2F, Bldg F, University Science Park

Tel: 0531-88819269

WeChat Official Account

Follow our WeChat subscription account for real-time updates and the latest in medical and biological research.


Business Email

E-mail: [email protected]

Copyright © 2026.Genelibs Jinan Honghui Biotechnology Co., Ltd. 鲁ICP备20007159号-2