The Molecular Weight and Aggregation of DNA.

Biophysical journal ·Vol. 1 ·No. 4 ·1961-03-00 ·Pages 301-15

Cavalieri LF, Deutsch JF, Rosenberg BH

Abstract

The effects of enzymatic attack and of shear during the isolation and deproteinization of DNA have been investigated. Different methods of disaggregating DNA have been studied, and conditions under which reaggregation can occur are discussed. It was found that shaking with chloroform-octanol does not degrade DNA from the seven sources studied; that light scattering yields valid weight-average molecular weights for these samples; and that, when disaggregated, the molecular weights of these samples are in the range 1.2-2.4 million and the length-to-mass ratios are high.

Authors & Affiliations

3 authors, click to expand affiliations / ORCID

Cavalieri L F

Deutsch J F

Rosenberg B H

References (7)

7 references, click to expand

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Proc Natl Acad Sci U S A. 1960 Apr;46(4):461-76 PMID: 16590628
The influence of protein on heterogeneity of DNA.
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A rapid method for preparing polymerized deoxyribonucleic acid from tissues, based on separation of nuclei.
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A pH transition in the sedimentation behavior of deoxyribonucleic acid.
J Biol Chem. 1960 Sep;235:2698-704 PMID: 14443871
The isolation of desoxyribonucleic acid from bacteriophages by an improved method.
J Biol Chem. 1954 Oct;210(2):877-84 PMID: 13211625
THE EFFECT OF HYDRODYNAMIC SHEAR ON THE DEOXYRIBONUCLEIC ACID FROM T(2) AND T(4) BACTERIOPHAGES.
Proc Natl Acad Sci U S A. 1959 Nov;45(11):1560-8 PMID: 16590544
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Article Info

Journal

Biophysical journal

Abbr.

Biophys J

ISSN

0006-3495

DOI

10.1016/s0006-3495(61)86890-2

Published

1961-03-00

Pages

301-15

Language

English

Region

United States

NLM ID

0370626

PMCID

PMC1366349

External Links

PubMed source DOI: 10.1016/s0006-3495(61)86890-2 PMC Full Text View journal details

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