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PMID: 19439494 Published · ppublish English Journal Article Research Support, N.I.H., Extramural

Genetic ablation of neural crest cell diversification.

Development (Cambridge, England) ·Vol. 136 ·No. 12 ·2009-06-00 ·Pages 1987-94

Arduini BL, Bosse KM, Henion PD

Abstract

The neural crest generates multiple cell types during embryogenesis but the mechanisms regulating neural crest cell diversification are incompletely understood. Previous studies using mutant zebrafish indicated that foxd3 and tfap2a function early and differentially in the development of neural crest sublineages. Here, we show that the simultaneous loss of foxd3 and tfap2a function in zebrafish foxd3(zdf10);tfap2a(low) double mutant embryos globally prevents the specification of developmentally distinct neural crest sublineages. By contrast, neural crest induction occurs independently of foxd3 and tfap2a function. We show that the failure of neural crest cell diversification in double mutants is accompanied by the absence of neural crest sox10 and sox9a/b gene expression, and that forced expression of sox10 and sox9a/b differentially rescues neural crest sublineage specification and derivative differentiation. These results demonstrate the functional necessity for foxd3 and tfap2a for neural crest sublineage specification and that this requirement is mediated by the synergistic regulation of the expression of SoxE family genes. Our results identify a genetic regulatory pathway functionally discrete from the process of neural crest induction that is required for the initiation of neural crest cell diversification during embryonic development.

MeSH Terms
Animals Cell Lineage/physiology Embryo, Nonmammalian/physiology Forkhead Transcription Factors/genetics,metabolism Gene Expression Regulation, Developmental Mutation Neural Crest/embryology,physiology SOX9 Transcription Factor/genetics,metabolism Transcription Factor AP-2/genetics,metabolism Zebrafish/embryology Zebrafish Proteins/genetics,metabolism
Chemicals
Forkhead Transcription Factors SOX9 Transcription Factor Sox9a protein, zebrafish Sox9b protein, zebrafish Transcription Factor AP-2 Zebrafish Proteins foxd3 protein, zebrafish tfap2a protein, zebrafish
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Arduini Brigitte L
Center for Molecular Neurobiology, Ohio State University, Columbus, OH 43210, USA.
Bosse Kevin M
Henion Paul D
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Article Info
Journal
Development (Cambridge, England)
Abbr.
Development
ISSN
0950-1991
DOI
10.1242/dev.033209
Published
2009-06-00
Epub
2009-00-13
Pages
1987-94
Language
English
Region
England
NLM ID
8701744
PMCID
PMC2685722
Subset
IM
Grants
NIGMS NIH HHS · GM076505 · United States
NINDS NIH HHS · P30-NS045758 · United States
External Links
PubMed source DOI: 10.1242/dev.033209 PMC Full Text View journal details
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