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PMID: 19458192 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Regulation of cell polarity through phosphorylation of Bni4 by Pho85 G1 cyclin-dependent kinases in Saccharomyces cerevisiae.

Molecular biology of the cell ·Vol. 20 ·No. 14 ·2009-07-00 ·Pages 3239-50

Zou J, Friesen H, Larson J, Huang D, Cox M, Tatchell K, Andrews B

Abstract

In the budding yeast Saccharomyces cerevisiae, the G1-specific cyclin-dependent kinases (Cdks) Cln1,2-Cdc28 and Pcl1,2-Pho85 are essential for ensuring that DNA replication and cell division are properly linked to cell polarity and bud morphogenesis. However, the redundancy of Cdks and cyclins means that identification of relevant Cdk substrates remains a significant challenge. We used array-based genetic screens (synthetic genetic array or SGA analysis) to dissect redundant pathways associated with G1 cyclins and identified Bni4 as a substrate of the Pcl1- and Pcl2-Pho85 kinases. BNI4 encodes an adaptor protein that targets several proteins to the bud neck. Deletion of BNI4 results in severe growth defects in the absence of the Cdc28 cyclins Cln1 and Cln2, and overexpression of BNI4 is toxic in yeast cells lacking the Pho85 cyclins Pcl1 and Pcl2. Phosphorylation of Bni4 by Pcl-Pho85 is necessary for its localization to the bud neck, and the bud neck structure can be disrupted by overexpressing BNI4 in pcl1Deltapcl2Delta mutant cells. Our data suggest that misregulated Bni4 may bind in an uncontrolled manner to an essential component that resides at the bud neck, causing catastrophic morphogenesis defects.

MeSH Terms
Cell Cycle Proteins/metabolism Cell Polarity Cyclin G Cyclin-Dependent Kinases/isolation & purification,metabolism Cyclins/genetics,metabolism Gene Dosage Genes, Fungal Mutation/genetics Phenotype Phosphorylation Protein Binding Protein Transport Saccharomyces cerevisiae/cytology,enzymology,genetics Saccharomyces cerevisiae Proteins/isolation & purification,metabolism Transcription Factors/metabolism
Chemicals
Bni4p protein, S cerevisiae Cell Cycle Proteins Cyclin G Cyclins PCL2 protein, S cerevisiae Saccharomyces cerevisiae Proteins Transcription Factors PCL1 protein, S cerevisiae Cyclin-Dependent Kinases PHO85 protein, S cerevisiae
Authors & Affiliations
7 authors, click to expand affiliations / ORCID
Zou Jian
Department of Molecular Genetics, University of Toronto, Toronto, Ontario M5S 3E1, Canada.
Friesen Helena
Larson Jennifer
Huang Dongqing
Cox Mike
Tatchell Kelly
Andrews Brenda
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Article Info
Journal
Molecular biology of the cell
Abbr.
Mol Biol Cell
ISSN
1939-4586
DOI
10.1091/mbc.e08-12-1255
Published
2009-07-00
Epub
2009-00-20
Pages
3239-50
Language
English
Region
United States
NLM ID
9201390
PMCID
PMC2710841
Subset
IM
External Links
PubMed source DOI: 10.1091/mbc.e08-12-1255 PMC Full Text View journal details
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