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PMID: 1945829 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S.

Molecular cloning of telomere-binding protein genes from Stylonychia mytilis.

Nucleic acids research ·Vol. 19 ·No. 20 ·1991-10-25 ·Pages 5515-8

Fang GW, Cech TR

Abstract

A telomere-binding protein heterodimer of 56 kDa (alpha) and 41 kDa (beta) subunits binds specifically to Oxytricha nova telomeres. Genes encoding both subunits have been cloned previously. Here we report molecular cloning and sequence analysis of the homologous genes in Stylonychia mytilis. The derived amino acid sequences were 79% identical for the alpha subunits and 77% identical for the beta subunits. Three repeats of a Leu/Ile heptad were found in each subunit, which might be involved in heterodimer formation. A 360 amino acid region of the Stylonychia mytilis alpha subunit was found to share weak sequence similarity with human vimentin, suggesting the possibility of a relationship between telomeres and intermediate filaments.

MeSH Terms

Amino Acid Sequence Animals Base Sequence Blotting, Southern Cloning, Molecular DNA, Protozoan/metabolism DNA-Binding Proteins/genetics,metabolism Molecular Sequence Data Restriction Mapping Sequence Alignment Sporadotrichina/genetics Telomere/metabolism

Chemicals

DNA, Protozoan DNA-Binding Proteins

Authors & Affiliations

2 authors, click to expand affiliations / ORCID

Fang G W

Department of Chemistry and Biochemistry, Howard Hughes Medical Institute, University of Colorado, Boulder 80309.

Cech T R

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Article Info

Journal

Nucleic acids research

Abbr.

Nucleic Acids Res

ISSN

0305-1048

DOI

10.1093/nar/19.20.5515

Published

1991-10-25

Pages

5515-8

Language

English

Region

England

NLM ID

0411011

PMCID

PMC328950

Subset

Grants

NIGMS NIH HHS · GM28039 · United States

Databases

GENBANK

S54217, S54227, S54228, S54230, X56461, X56854, X58431, X60824, X61748, X61749

External Links

PubMed source DOI: 10.1093/nar/19.20.5515 PMC Full Text View journal details

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Molecular cloning of telomere-binding protein genes from Stylonychia mytilis.

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