Home LiteratureArticle Details
PMID: 19481515 Published · ppublish English Journal Article Research Support, N.I.H., Extramural Research Support, Non-U.S. Gov't

Generation of human induced pluripotent stem cells by direct delivery of reprogramming proteins.

Cell stem cell ·Vol. 4 ·No. 6 ·2009-06-05 ·Pages 472-6

Kim D, Kim CH, Moon JI, Chung YG, Chang MY, Han BS, Ko S, Yang E, Cha KY, Lanza R, Kim KS

Abstract

暂无摘要

MeSH Terms
Cell Dedifferentiation DNA-Binding Proteins/physiology Humans Kruppel-Like Factor 4 Kruppel-Like Transcription Factors Methods Octamer Transcription Factor-3 Pluripotent Stem Cells/cytology Proto-Oncogene Proteins c-myc SOXB1 Transcription Factors Transduction, Genetic
Chemicals
DNA-Binding Proteins Kruppel-Like Factor 4 Kruppel-Like Transcription Factors MYC protein, human Octamer Transcription Factor-3 POU5F1 protein, human Proto-Oncogene Proteins c-myc SOX2 protein, human SOXB1 Transcription Factors
Authors & Affiliations
11 authors, click to expand affiliations / ORCID
Kim Dohoon
Molecular Neurobiology Laboratory, Department of Psychiatry and McLean Hospital, Harvard Medical School, 115 Mill Street, Belmont, MA 02478, USA.
Kim Chun-Hyung
Moon Jung-Il
Chung Young-Gie
Chang Mi-Yoon
Han Baek-Soo
Ko Sanghyeok
Yang Eungi
Cha Kwang Yul
Lanza Robert
Kim Kwang-Soo
References (30)
30 references, click to expand
  1. Tat protein from human immunodeficiency virus forms a metal-linked dimer.
    Science. 1988 Apr 1;240(4848):70-3 PMID: 2832944
  2. Generation of rat and human induced pluripotent stem cells by combining genetic reprogramming and chemical inhibitors.
    Cell Stem Cell. 2009 Jan 9;4(1):16-9 PMID: 19097958
  3. Cellular uptake of the tat protein from human immunodeficiency virus.
    Cell. 1988 Dec 23;55(6):1189-93 PMID: 2849510
  4. Treatment of sickle cell anemia mouse model with iPS cells generated from autologous skin.
    Science. 2007 Dec 21;318(5858):1920-3 PMID: 18063756
  5. Induction of pluripotent stem cells from primary human fibroblasts with only Oct4 and Sox2.
    Nat Biotechnol. 2008 Nov;26(11):1269-75 PMID: 18849973
  6. Parkinson's disease patient-derived induced pluripotent stem cells free of viral reprogramming factors.
    Cell. 2009 Mar 6;136(5):964-77 PMID: 19269371
  7. Strategies and new developments in the generation of patient-specific pluripotent stem cells.
    Cell Stem Cell. 2007 Jun 7;1(1):39-49 PMID: 18371333
  8. Viable offspring derived from fetal and adult mammalian cells.
    Nature. 1997 Feb 27;385(6619):810-3 PMID: 9039911
  9. Generation of induced pluripotent stem cells using recombinant proteins.
    Cell Stem Cell. 2009 May 8;4(5):381-4 PMID: 19398399
  10. Neurons derived from reprogrammed fibroblasts functionally integrate into the fetal brain and improve symptoms of rats with Parkinson's disease.
    Proc Natl Acad Sci U S A. 2008 Apr 15;105(15):5856-61 PMID: 18391196
  11. piggyBac transposition reprograms fibroblasts to induced pluripotent stem cells.
    Nature. 2009 Apr 9;458(7239):766-70 PMID: 19252478
  12. Generation of germline-competent induced pluripotent stem cells.
    Nature. 2007 Jul 19;448(7151):313-7 PMID: 17554338
  13. Induction of pluripotent stem cells from mouse embryonic fibroblasts by Oct4 and Klf4 with small-molecule compounds.
    Cell Stem Cell. 2008 Nov 6;3(5):568-74 PMID: 18983970
  14. Reprogramming of human somatic cells to pluripotency with defined factors.
    Nature. 2008 Jan 10;451(7175):141-6 PMID: 18157115
  15. Induction of pluripotent stem cells from mouse embryonic and adult fibroblast cultures by defined factors.
    Cell. 2006 Aug 25;126(4):663-76 PMID: 16904174
  16. Development of human cloned blastocysts following somatic cell nuclear transfer with adult fibroblasts.
    Stem Cells. 2008 Feb;26(2):485-93 PMID: 18202077
  17. Human induced pluripotent stem cells free of vector and transgene sequences.
    Science. 2009 May 8;324(5928):797-801 PMID: 19325077
  18. Directly reprogrammed fibroblasts show global epigenetic remodeling and widespread tissue contribution.
    Cell Stem Cell. 2007 Jun 7;1(1):55-70 PMID: 18371336
  19. In vitro reprogramming of fibroblasts into a pluripotent ES-cell-like state.
    Nature. 2007 Jul 19;448(7151):318-24 PMID: 17554336
  20. Induced pluripotent stem cells generated without viral integration.
    Science. 2008 Nov 7;322(5903):945-9 PMID: 18818365
  21. Reprogramming of human somatic cells using human and animal oocytes.
    Cloning Stem Cells. 2009 Jun;11(2):213-23 PMID: 19186982
  22. The cationic cell-penetrating peptide CPP(TAT) derived from the HIV-1 protein TAT is rapidly transported into living fibroblasts: optical, biophysical, and metabolic evidence.
    Biochemistry. 2005 Jan 11;44(1):138-48 PMID: 15628854
  23. The design, synthesis, and evaluation of molecules that enable or enhance cellular uptake: peptoid molecular transporters.
    Proc Natl Acad Sci U S A. 2000 Nov 21;97(24):13003-8 PMID: 11087855
  24. A fresh look at iPS cells.
    Cell. 2009 Apr 3;137(1):13-7 PMID: 19345179
  25. Generation of mouse induced pluripotent stem cells without viral vectors.
    Science. 2008 Nov 7;322(5903):949-53 PMID: 18845712
  26. Induction of pluripotent stem cells from adult human fibroblasts by defined factors.
    Cell. 2007 Nov 30;131(5):861-72 PMID: 18035408
  27. Virus-free induction of pluripotency and subsequent excision of reprogramming factors.
    Nature. 2009 Apr 9;458(7239):771-5 PMID: 19252477
  28. Nuclear delivery of macromolecules: barriers and carriers.
    Adv Drug Deliv Rev. 2005 Feb 28;57(4):505-27 PMID: 15722161
  29. Induced pluripotent stem cell lines derived from human somatic cells.
    Science. 2007 Dec 21;318(5858):1917-20 PMID: 18029452
  30. Delivery of macromolecules using arginine-rich cell-penetrating peptides: ways to overcome endosomal entrapment.
    AAPS J. 2009 Mar;11(1):13-22 PMID: 19125334
Article Info
Journal
Cell stem cell
Abbr.
Cell Stem Cell
ISSN
1875-9777
DOI
10.1016/j.stem.2009.05.005
Published
2009-06-05
Epub
2009-00-28
Pages
472-6
Language
English
Region
United States
NLM ID
101311472
PMCID
PMC2705327
Subset
IM
Grants
NIMH NIH HHS · R01 MH048866 · United States
NIMH NIH HHS · R01 MH048866-17 · United States
NINDS NIH HHS · R01 NS084869 · United States
NIMH NIH HHS · R29 MH048866 · United States
NIDCD NIH HHS · R01 DC006501 · United States
NIDCD NIH HHS · DC 006501 · United States
NIDCD NIH HHS · R01 DC006501-05 · United States
NIMH NIH HHS · MH48866 · United States
Databases
GEO
GSE16093, GSM402707, GSM402708, GSM402717, GSM402752, GSM402806
External Links
PubMed source DOI: 10.1016/j.stem.2009.05.005 PMC Full Text View journal details
Analysis Services
Analysis Services

Contact

No. 2 Wenbo Road, Zhangqiu District, Jinan, Shandong

Qilu Normal University · Genelibs Bioinformatics Lab

750 Shunhua Rd, Jinan

2F, Bldg F, University Science Park

Tel: 0531-88819269

WeChat Official Account

Follow our WeChat subscription account for real-time updates and the latest in medical and biological research.


Business Email

E-mail: [email protected]

Copyright © 2026.Genelibs Jinan Honghui Biotechnology Co., Ltd. 鲁ICP备20007159号-2