Electrophoretic analysis of the molecular weight of murine mammary tumor virus RNA.

Journal of virology ·Vol. 22 ·No. 3 ·1977-06-00 ·Pages 822-5

Dion AS, Heine UI, Pomenti AA, Korb J, Weber GH

Abstract

Molecular weight determinations of native and subunit RNAs of murine mammary tumor virus (MuMTV), a type B oncornavirus, were performed by polyacrylamide gel electrophoresis and compared with molecular weights of well-characterized avian cellular RNAs and tobacco mosaic virus RNA. From extrapolations of semilog plots of the molecular weights of the standard RNAs versus relative electrophoretic mobilities and Ferguson plots, the subunit and native RNAs of MuMTV were found to possess molecular weights of 2.93 X 10(6) and 6.45 X 10(6), respectively. These data support the assumption that two subunit molecules comprise the native RNA of MuMTV.

MeSH Terms

Animals Birds Electrophoresis, Polyacrylamide Gel Mammary Tumor Virus, Mouse/analysis Microscopy, Electron Molecular Weight RNA/analysis RNA, Viral/analysis Tobacco Mosaic Virus/analysis

Chemicals

RNA, Viral RNA

Authors & Affiliations

5 authors, click to expand affiliations / ORCID

Dion A S

Heine U I

Pomenti A A

Korb J

Weber G H

References (13)

13 references, click to expand

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Article Info

Journal

Journal of virology

Abbr.

J Virol

ISSN

0022-538X

DOI

10.1128/JVI.22.3.822-825.1977

Published

1977-06-00

Pages

822-5

Language

English

Region

United States

NLM ID

0113724

PMCID

PMC515781

Subset

External Links

PubMed source DOI: 10.1128/JVI.22.3.822-825.1977 PMC Full Text View journal details

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Electrophoretic analysis of the molecular weight of murine mammary tumor virus RNA.

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