The reduction of Pseudomonas cytochrome c551 oxidase by chromous ions.

The Biochemical journal ·Vol. 163 ·No. 3 ·1977-06-01 ·Pages 629-32

Barber D, Parr SR, Greenwood C

Abstract

The reduction of cytochrome c551 oxidase from Pseudomonas aeruginosa by Cr2+ ions was followed in the stopped-flow apparatus at a number of wavelengths. The c-haem reduction proceeded in a biphasic fashion with second-order rate constants of 2.6 X 10(5)M-1-S-1 and 4.8 X 10(4)M-1-S-1 at 25 degrees C, whereas the biphasic reduction of the d1-haem appeared to be independent of reductant concentration with rate constants of approx. 1.0S-1 and 0.25S-1 respectively. The kinetically determined difference spectra (reduced minus oxidized) for the c- and d1-haems are presented.

MeSH Terms

Chromium Electron Transport Complex IV/metabolism Kinetics Oxidation-Reduction Pseudomonas aeruginosa/enzymology

Chemicals

Chromium Electron Transport Complex IV

Authors & Affiliations

3 authors, click to expand affiliations / ORCID

Barber D

Parr S R

Greenwood C

References (9)

9 references, click to expand

Purification and properties of cytochrome oxidase from Pseudomonas aeruginosa.
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A purification procedure for the soluble cytochrome oxidase and some other respiratory proteins from Pseudomonas aeruginosa.
Biochem J. 1976 Aug 1;157(2):423-30 PMID: 183750
Some spectral and steady-state kinetic properties of Pseudomonas cytochrome oxidase.
Biochem J. 1976 Aug 1;157(2):431-8 PMID: 183751
Kinetic studies on mammalian cytochrome c modified with 2-hydroxy-5-hydroxy-5-nitrobenzyl bromide.
Biochem J. 1975 Jul;149(1):179-85 PMID: 242320
Apparatus for rapid and sensitive spectrophotometry.
Biochem J. 1964 Apr;91(1):161-71 PMID: 5833381
A new purification procedure and molecular properties of Pseudomonas cytochrome oxidase.
Biochim Biophys Acta. 1972 Sep 20;275(3):308-18 PMID: 4341773
Electron transfer reactions in biological systems: the reduction of ferricytochrome c by chromous ions.
Proc Natl Acad Sci U S A. 1974 Apr;71(4):1408-12 PMID: 4364536
The reduction of carboxymethyl-cytochrome c by chromous ions.
Biochem J. 1974 Aug;141(2):455-61 PMID: 4375979
A study of the mechanism of electron transfer in cytochrome c. Chromium as a probe.
J Biol Chem. 1969 Dec 25;244(24):6619-25 PMID: 4311913

Article Info

Journal

The Biochemical journal

Abbr.

Biochem J

ISSN

0264-6021

DOI

10.1042/bj1630629

Published

1977-06-01

Pages

629-32

Language

English

Region

England

NLM ID

2984726R

PMCID

PMC1164743

Subset

External Links

PubMed source DOI: 10.1042/bj1630629 PMC Full Text View journal details

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The reduction of Pseudomonas cytochrome c551 oxidase by chromous ions.

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