Home LiteratureArticle Details
PMID: 19577919 Published · ppublish English Journal Article Research Support, N.I.H., Extramural Research Support, Non-U.S. Gov't Review

Mass spectrometry in the analysis of N-linked and O-linked glycans.

Current opinion in structural biology ·Vol. 19 ·No. 5 ·2009-10-00 ·Pages 498-506

North SJ, Hitchen PG, Haslam SM, Dell A

Abstract

Mass spectrometry (MS) continues to play a vital role in defining the structures of N-glycans and O-glycans in glycoproteins via glycomic and glycoproteomic methodologies. The former seeks to define the total N-glycan and/or O-glycan repertoire in a biological sample whilst the latter is concerned with the analysis of glycopeptides. Recent technical developments have included improvements in tandem mass spectrometry (MS/MS and MS(n)) sequencing methodologies, more sensitive methods for analysing sulfated and polysialylated glycans and better procedures for defining the sites of O-glycosylation. New tools have been introduced to assist data handling and publicly accessible databases are being populated with glycomics data. Progress is exemplified by recent research in the fields of glycoimmunology, reproductive glycobiology, stem cells, bacterial glycosylation and non-mucin O-glycosylation.

MeSH Terms
Animals Antigens/chemistry,immunology Glycomics Humans Mass Spectrometry/methods Polysaccharides/antagonists & inhibitors,chemistry,immunology Proteomics Stem Cells/chemistry
Chemicals
Antigens Polysaccharides
Authors & Affiliations
4 authors, click to expand affiliations / ORCID
North Simon J
Division of Molecular Biosciences, Faculty of Natural Sciences, Imperial College London, London SW7 2AZ, UK.
Hitchen Paul G
Haslam Stuart M
Dell Anne
References (50)
50 references, click to expand
  1. Dystroglycan glycosylation and its role in alpha-dystroglycanopathies.
    Acta Myol. 2007 Dec;26(3):165-70 PMID: 18646566
  2. Protein glycosylation in bacterial mucosal pathogens.
    Nat Rev Microbiol. 2005 Mar;3(3):225-37 PMID: 15738950
  3. Neisseria gonorrhoeae O-linked pilin glycosylation: functional analyses define both the biosynthetic pathway and glycan structure.
    Mol Microbiol. 2007 Aug;65(3):607-24 PMID: 17608667
  4. Mass spectrometric analysis of N- and O-glycosylation of tissues and cells.
    Curr Opin Struct Biol. 2006 Oct;16(5):584-91 PMID: 16938453
  5. Structural characterisation of neutrophil glycans by ultra sensitive mass spectrometric glycomics methodology.
    Glycoconj J. 2009 Nov;26(8):975-86 PMID: 18587645
  6. Rumi is a CAP10 domain glycosyltransferase that modifies Notch and is required for Notch signaling.
    Cell. 2008 Jan 25;132(2):247-58 PMID: 18243100
  7. Expression of bisecting type and Lewisx/Lewisy terminated N-glycans on human sperm.
    J Biol Chem. 2007 Dec 14;282(50):36593-602 PMID: 17913713
  8. Cross-talk between GlcNAcylation and phosphorylation: site-specific phosphorylation dynamics in response to globally elevated O-GlcNAc.
    Proc Natl Acad Sci U S A. 2008 Sep 16;105(37):13793-8 PMID: 18779572
  9. The role of informatics in glycobiology research with special emphasis on automatic interpretation of MS spectra.
    Biochim Biophys Acta. 2006 Apr;1760(4):568-77 PMID: 16459020
  10. 7th HUPO World Congress: the human disease glycomics/proteomics initiative (HGPI) session 17 August 2008, Amsterdam, The Netherlands.
    Proteomics. 2009 Apr;9(7):1738-41 PMID: 19294704
  11. O-fucosylation is required for ADAMTS13 secretion.
    J Biol Chem. 2007 Jun 8;282(23):17014-23 PMID: 17395589
  12. Not just for Eukarya anymore: protein glycosylation in Bacteria and Archaea.
    Curr Opin Struct Biol. 2008 Oct;18(5):544-50 PMID: 18694827
  13. A complex, but uniform O-glycosylation of the human MUC2 mucin from colonic biopsies analyzed by nanoLC/MSn.
    Glycobiology. 2009 Jul;19(7):756-66 PMID: 19321523
  14. A PGC-1alpha-O-GlcNAc transferase complex regulates FoxO transcription factor activity in response to glucose.
    J Biol Chem. 2009 Feb 20;284(8):5148-57 PMID: 19103600
  15. Characterization of oligo- and polysialic acids by MALDI-TOF-MS.
    Anal Chem. 2007 Sep 15;79(18):7161-9 PMID: 17705556
  16. Site-specific GlcNAcylation of human erythrocyte proteins: potential biomarker(s) for diabetes.
    Diabetes. 2009 Feb;58(2):309-17 PMID: 18984734
  17. Critical functions of N-glycans in L-selectin-mediated lymphocyte homing and recruitment.
    Nat Immunol. 2007 Apr;8(4):409-18 PMID: 17334369
  18. Effects of differential glycosylation of glycodelins on lymphocyte survival.
    J Biol Chem. 2009 May 29;284(22):15084-96 PMID: 19240032
  19. C-Mannosylated peptides derived from the thrombospondin type 1 repeat enhance lipopolysaccharide-induced signaling in macrophage-like RAW264.7 cells.
    Glycobiology. 2007 Sep;17(9):1015-28 PMID: 17602137
  20. Glycosyltransferase function in core 2-type protein O glycosylation.
    Mol Cell Biol. 2009 Jul;29(13):3770-82 PMID: 19349303
  21. O-glycosylation pattern of CD24 from mouse brain.
    Biol Chem. 2009 Jul;390(7):627-45 PMID: 19284289
  22. Frontiers in glycomics: bioinformatics and biomarkers in disease. An NIH white paper prepared from discussions by the focus groups at a workshop on the NIH campus, Bethesda MD (September 11-13, 2006).
    Proteomics. 2008 Jan;8(1):8-20 PMID: 18095367
  23. Role of unusual O-glycans in intercellular signaling.
    Int J Biochem Cell Biol. 2009 May;41(5):1011-24 PMID: 18952191
  24. Integrated mass spectrometric strategy for characterizing the glycans from glycosphingolipids and glycoproteins: direct identification of sialyl Le(x) in mice.
    Glycobiology. 2007 Jun;17(6):646-54 PMID: 17341505
  25. Probing the dynamics of O-GlcNAc glycosylation in the brain using quantitative proteomics.
    Nat Chem Biol. 2007 Jun;3(6):339-48 PMID: 17496889
  26. GlycoWorkbench: a tool for the computer-assisted annotation of mass spectra of glycans.
    J Proteome Res. 2008 Apr;7(4):1650-9 PMID: 18311910
  27. Cross-talk between GlcNAcylation and phosphorylation: roles in insulin resistance and glucose toxicity.
    Am J Physiol Endocrinol Metab. 2008 Jul;295(1):E17-28 PMID: 18445751
  28. Peters Plus syndrome is a new congenital disorder of glycosylation and involves defective Omicron-glycosylation of thrombospondin type 1 repeats.
    J Biol Chem. 2008 Mar 21;283(12):7354-60 PMID: 18199743
  29. Identification of novel carbohydrate modifications on Campylobacter jejuni 11168 flagellin using metabolomics-based approaches.
    FEBS J. 2009 Feb;276(4):1014-23 PMID: 19154343
  30. The glycans of stem cells.
    Curr Opin Chem Biol. 2007 Aug;11(4):373-80 PMID: 17681848
  31. An introduction to bioinformatics for glycomics research.
    PLoS Comput Biol. 2008 May 30;4(5):e1000075 PMID: 18516240
  32. Glycan family analysis for deducing N-glycan topology from single MS.
    Bioinformatics. 2009 Feb 1;25(3):365-71 PMID: 19073587
  33. Dendritic cell maturation results in pronounced changes in glycan expression affecting recognition by siglecs and galectins.
    J Immunol. 2007 Dec 15;179(12):8216-24 PMID: 18056365
  34. The impact of glycosylation on the biological function and structure of human immunoglobulins.
    Annu Rev Immunol. 2007;25:21-50 PMID: 17029568
  35. Identification of isomeric N-glycan structures by mass spectrometry with 157 nm laser-induced photofragmentation.
    J Am Soc Mass Spectrom. 2008 Jul;19(7):1027-40 PMID: 18487060
  36. The diversity of O-linked glycans expressed during Drosophila melanogaster development reflects stage- and tissue-specific requirements for cell signaling.
    J Biol Chem. 2008 Oct 31;283(44):30385-400 PMID: 18725413
  37. N-glycan structures and associated gene expression reflect the characteristic N-glycosylation pattern of human hematopoietic stem and progenitor cells.
    Exp Hematol. 2007 Aug;35(8):1279-92 PMID: 17662891
  38. Characterizing the glycome of the mammalian immune system.
    Immunol Cell Biol. 2008 Oct;86(7):564-73 PMID: 18725885
  39. Bacterial glycoproteomics.
    Microbiology (Reading). 2006 Jun;152(Pt 6):1575-1580 PMID: 16735721
  40. Sialyl-Lewis(x) on P-selectin glycoprotein ligand-1 is regulated during differentiation and maturation of dendritic cells: a mechanism involving the glycosyltransferases C2GnT1 and ST3Gal I.
    J Immunol. 2007 Nov 1;179(9):5701-10 PMID: 17947642
  41. Recapitulation of IVIG anti-inflammatory activity with a recombinant IgG Fc.
    Science. 2008 Apr 18;320(5874):373-6 PMID: 18420934
  42. Quantitation by isobaric labeling: applications to glycomics.
    J Proteome Res. 2008 Jan;7(1):367-74 PMID: 18047270
  43. Anti-inflammatory activity of immunoglobulin G resulting from Fc sialylation.
    Science. 2006 Aug 4;313(5787):670-3 PMID: 16888140
  44. Ebola sGP--the first viral glycoprotein shown to be C-mannosylated.
    Virology. 2007 Nov 10;368(1):83-90 PMID: 17659315
  45. Towards GAG glycomics: analysis of highly sulfated heparins by MALDI-TOF mass spectrometry.
    Glycobiology. 2007 Sep;17(9):972-82 PMID: 17623722
  46. Glycomics and proteomics analyses of mouse uterine luminal fluid revealed a predominance of Lewis Y and X epitopes on specific protein carriers.
    Mol Cell Proteomics. 2009 Feb;8(2):325-42 PMID: 18941134
  47. Glycomics of bone marrow-derived mesenchymal stem cells can be used to evaluate their cellular differentiation stage.
    Glycoconj J. 2009 Apr;26(3):367-84 PMID: 19037724
  48. A tetraantennary glycan with bisecting N-acetylglucosamine and the Sd(a) antigen is the predominant N-glycan on bovine pregnancy-associated glycoproteins.
    Glycobiology. 2008 Jan;18(1):42-52 PMID: 17951374
  49. Elucidation of O-glycosylation structures of the beta-amyloid precursor protein by liquid chromatography-mass spectrometry using electron transfer dissociation and collision induced dissociation.
    J Proteome Res. 2009 Feb;8(2):631-42 PMID: 19093876
  50. Salivary MUC7 is a major carrier of blood group I type O-linked oligosaccharides serving as the scaffold for sialyl Lewis x.
    Glycobiology. 2009 Mar;19(3):288-300 PMID: 19043084
Article Info
Journal
Current opinion in structural biology
Abbr.
Curr Opin Struct Biol
ISSN
1879-033X
DOI
10.1016/j.sbi.2009.05.005
Published
2009-10-00
Epub
2009-00-03
Pages
498-506
Language
English
Region
England
NLM ID
9107784
PMCID
PMC2965404
Subset
IM
Grants
NIGMS NIH HHS · U54 GM062116-09 · United States
Biotechnology and Biological Sciences Research Council · BBF0083091 · United Kingdom
Wellcome Trust · United Kingdom
NIGMS NIH HHS · GM62116 · United States
NIGMS NIH HHS · U54 GM062116 · United States
Biotechnology and Biological Sciences Research Council · BBC5196701 · United Kingdom
Biotechnology and Biological Sciences Research Council · B19088 · United Kingdom
External Links
PubMed source DOI: 10.1016/j.sbi.2009.05.005 PMC Full Text View journal details
Analysis Services
Analysis Services

Contact

No. 2 Wenbo Road, Zhangqiu District, Jinan, Shandong

Qilu Normal University · Genelibs Bioinformatics Lab

750 Shunhua Rd, Jinan

2F, Bldg F, University Science Park

Tel: 0531-88819269

WeChat Official Account

Follow our WeChat subscription account for real-time updates and the latest in medical and biological research.


Business Email

E-mail: [email protected]

Copyright © 2026.Genelibs Jinan Honghui Biotechnology Co., Ltd. 鲁ICP备20007159号-2