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PMID: 195865 Published · ppublish English Journal Article Research Support, U.S. Gov't, Non-P.H.S. Research Support, U.S. Gov't, P.H.S.

Isolation and characterization of MMS-sensitive mutants of Saccharomyces cerevisiae.

Genetics ·Vol. 86 ·No. 1 ·1977-05-00 ·Pages 33-55

Prakash L, Prakash S

Abstract

We have isolated mutants sensitive to methyl methanesulfonate (MMS) in Saccharomyces cerevisiae. Alleles of rad1, rad4, rad52, rad55 and rad57 were found amoung these mms mutants. Twenty-nine of the mms mutants which complement the existing radiation-sensitive (rad and rev) mutants belong to 22 new complementation groups. Mutants from five complementation groups are sensitive only to MMS. Mutants of 11 complementation groups are sensitive to UV or X rays in addition to MMS, mutants of six complementation groups are sensitive to all three agents. The cross-sensitivities of these mms mutants to UV and X rays are discussed in terms of their possible involvement in DNA repair. Sporulation is reduced or absent in homozygous diploids of mms mutants from nine complementation groups.

MeSH Terms
Alleles DNA Repair Genetic Complementation Test Mesylates/pharmacology Methyl Methanesulfonate/pharmacology Mutation Saccharomyces cerevisiae/drug effects,physiology,radiation effects Spores, Fungal Ultraviolet Rays X-Rays
Chemicals
Mesylates Methyl Methanesulfonate
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Prakash L
Prakash S
References (20)
20 references, click to expand
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Article Info
Journal
Genetics
Abbr.
Genetics
ISSN
0016-6731
DOI
10.1093/genetics/86.1.33
Published
1977-05-00
Pages
33-55
Language
English
Region
United States
NLM ID
0374636
PMCID
PMC1213670
Subset
IM
External Links
PubMed source DOI: 10.1093/genetics/86.1.33 PMC Full Text View journal details
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